Sanguansin S, Petmitr S, Punyarit P, Vorasubin V, Weerapradist W, Surarit R
Department of Physiology and Biochemistry, Mahidol University, Bangkok, Thailand.
J Exp Clin Cancer Res. 2006 Jun;25(2):251-7.
Oral cancer, one of the ten most widespread cancers in Thailand, is a major public health problem. The aim of the study was to assess hMSH2 and hMLH1 gene mutations, microsatellite DNA alterations, and investigate the association between these alterations and clinicopathological features of oral squamous cell carcinomas (SCC) in a sample of Thai patients. Microsatellite alterations at D2S391, D3S647, D17S513, and D17S520 were detected at a frequency of 40.6%. Among these alterations, 12.5% exhibited loss of heterozygosity (LOH) at D3S647 and D17S513, while 34.4% exhibited microsatellite instability (MI) at D2S391, D17S513, and D17S520. Polymorphic change in the intronic region of hMSH2 at IVS 1 nt 211+9, c-->g was observed in 50% of cases. Significant correlation was observed between IVS 1 nt 211+9 polymorphism and the recurrence status of the patients (p = 0.030, OR = 10.67). This study demonstrated that the polymorphism of hMSH2 at IVS 1 nt 211+9 (c-->g) was associated with oral cancer recurrence status and could be used as a biomarker for prognosis and follow-up treatment of oral cancer.
口腔癌是泰国十大最常见癌症之一，是一个重大的公共卫生问题。本研究的目的是评估hMSH2和hMLH1基因突变、微卫星DNA改变，并调查这些改变与泰国患者样本中口腔鳞状细胞癌（SCC）临床病理特征之间的关联。在D2S391、D3S647、D17S513和D17S520处检测到微卫星改变的频率为40.6%。在这些改变中，12.5%在D3S647和D17S513处表现出杂合性缺失（LOH），而34.4%在D2S391、D17S513和D17S520处表现出微卫星不稳定性（MI）。在50%的病例中观察到hMSH2内含子区域IVS 1 nt 211+9处发生c-->g的多态性变化。观察到IVS 1 nt 211+9多态性与患者的复发状态之间存在显著相关性（p = 0.030，OR = 10.67）。本研究表明，hMSH2在IVS 1 nt 211+9（c-->g）处的多态性与口腔癌复发状态相关，可作为口腔癌预后和后续治疗的生物标志物。
