FCD1编码的蛋白与尖孢镰刀菌中的纤维二糖：醌氧化还原酶同源。

FCD1 encoding protein homologous to cellobiose: quinone oxidoreductase in Fusarium oxysporum.

作者信息

Kawabe Masato, Yoshida Takanobu, Teraoka Tohru, Arie Tsutomu

机构信息

United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo, Japan.

出版信息

Gene. 2006 Nov 1;382:100-10. doi: 10.1016/j.gene.2006.06.015. Epub 2006 Jul 6.

DOI:10.1016/j.gene.2006.06.015

PMID:16919403

Abstract

We had cloned and characterized a gene from Fusarium oxysporum designated FCD1, encoding a putative cellobiose: quinone oxidoreductase (CBQ) which is a member of the extracellular redox enzyme family and also a member of glucose-methanol-choline (GMC) oxidoreductases. CBQ is known to be a free flavin domain of a cellobiose dehydrogenase (CDH) generated by proteolysis, but FCD1 gene encodes CBQ directly. In a phylogenetic tree of amino acid sequences of FCD1, GMC oxidoreductases and hypothetical GMC oxidoreductases, FCD1 clustered together with flavin domains (CBQs) of CDHs and putative proteins with unknown function of ascomycetes. FCD1-disruptants showed no reduction in virulence toward tomato and no obvious morphological effects such as production of conidia and mycelial growth as compared to the wild type strain, suggesting that FCD1 is not essential for virulence and vigor in F. oxysporum.

摘要

我们已经从尖孢镰刀菌中克隆并鉴定了一个名为FCD1的基因，该基因编码一种假定的纤维二糖：醌氧化还原酶（CBQ），它是细胞外氧化还原酶家族的成员，也是葡萄糖-甲醇-胆碱（GMC）氧化还原酶的成员。已知CBQ是通过蛋白水解产生的纤维二糖脱氢酶（CDH）的游离黄素结构域，但FCD1基因直接编码CBQ。在FCD1、GMC氧化还原酶和假定的GMC氧化还原酶的氨基酸序列系统发育树中，FCD1与CDH的黄素结构域（CBQ）以及子囊菌中功能未知的假定蛋白质聚集在一起。与野生型菌株相比，FCD1缺失突变体对番茄的毒力没有降低，并且在分生孢子产生和菌丝生长等方面没有明显的形态学影响，这表明FCD1对于尖孢镰刀菌的毒力和活力不是必需的。