Chang Jung-Su, Dheda Keertan, Huggett Jim F, Kim Louise U, Zumla Alimuddin, Rook Graham A W
Centre for Infectious Diseases and International Health, Royal Free and University College Medical School, 46 Cleveland Street, London W1T 4JF, United Kingdom.
Immunol Lett. 2006 Aug 15;106(2):194-7. doi: 10.1016/j.imlet.2006.06.001. Epub 2006 Jun 27.
When IL-4 mRNA was distinguished from mRNA encoding its antagonist, the splice variant IL-4delta2, it was found to correlate directly with expression of TLR2 in fresh peripheral blood mononuclear cells (PBMCs) from normal donors (p=0.0013). Similarly IL-4 mRNA was high when TLR2 mRNA was abundant compared to levels of mRNA encoding its heterodimerisation partners TLR1 (p=0.0007) or TLR6 (p=0.0007). IL-4delta2 tended to show the reverse effect; IL-4delta2 mRNA was high when TLR2 was low relative to TLR1 (p=0.001). When subpopulations of the PBMCs were examined these relationships were found to be restricted to the CD3+ cells. The CD3+ cell population from 5 of 10 donors had detectable TLR2 mRNA. When levels of TLR1, IL-4 and IFN-gamma mRNA were assayed in the TLR2(low) and TLR2(high) CD3+ cells, it was found that IL-4 mRNA was restricted to the TLR2(high) T cells (p=0.007) while TLR1 was higher in the TLR2(low) T cells (p=0.015). IFN-gamma was also somewhat increased in the TLR2(low) (ns). None of these correlations with TLR mRNA expression levels were found in similar samples from tuberculosis patients, or when similar analyses were performed with data for IL-10 mRNA in cells from the same donors. We conclude that in T cell populations from normal donors, expression of IL-4 (but not of its antagonist, IL-4delta2, or of IL-10) is associated with high TLR2 and low TLR1.
当将白细胞介素-4（IL-4）信使核糖核酸（mRNA）与其编码拮抗剂的剪接变体IL-4δ2的mRNA区分开时，发现其与正常供体新鲜外周血单个核细胞（PBMC）中Toll样受体2（TLR2）的表达直接相关（p = 0.0013）。同样，与编码其异二聚体伙伴TLR1（p = 0.0007）或TLR6（p = 0.0007）的mRNA水平相比，当TLR2 mRNA丰富时，IL-4 mRNA水平较高。IL-4δ2则倾向于表现出相反的效应；相对于TLR1，当TLR2水平较低时，IL-4δ2 mRNA水平较高（p = 0.001）。当检查PBMC的亚群时，发现这些关系仅限于CD3 +细胞。10名供体中有5名的CD3 +细胞群体可检测到TLR2 mRNA。当在TLR2（低）和TLR2（高）的CD3 +细胞中检测TLR1、IL-4和γ干扰素（IFN-γ）mRNA水平时，发现IL-4 mRNA仅限于TLR2（高）的T细胞（p = 0.007），而TLR1在TLR2（低）的T细胞中更高（p = 0.015）。TLR2（低）的IFN-γ也略有增加（无统计学意义）。在结核病患者的类似样本中，未发现这些与TLR mRNA表达水平的相关性，或者对来自相同供体的细胞中IL-10 mRNA数据进行类似分析时也未发现。我们得出结论，在正常供体的T细胞群体中，IL-4（而非其拮抗剂IL-4δ2或IL-10）的表达与高TLR2和低TLR1相关。
