Goulas Estelle, Schubert Maria, Kieselbach Thomas, Kleczkowski Leszek A, Gardeström Per, Schröder Wolfgang, Hurry Vaughan
Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, S-901 87 Umeå, Sweden.
Plant J. 2006 Sep;47(5):720-34. doi: 10.1111/j.1365-313X.2006.02821.x.
Cold acclimation and over-wintering by herbaceous plants are energetically expensive and are dependent on functional plastid metabolism. To understand how the stroma and the lumen proteomes adapt to low temperatures, we have taken a proteomic approach (difference gel electrophoresis) to identify proteins that changed in abundance in Arabidopsis chloroplasts during cold shock (1 day), and short- (10 days) and long-term (40 days) acclimation to 5 degrees C. We show that cold shock (1 day) results in minimal change in the plastid proteomes, while short-term (10 days) acclimation results in major changes in the stromal but few changes in the lumen proteome. Long-term acclimation (40 days) results in modulation of the proteomes of both compartments, with new proteins appearing in the lumen and further modulations in protein abundance occurring in the stroma. We identify 43 differentially displayed proteins that participate in photosynthesis, other plastid metabolic functions, hormone biosynthesis and stress sensing and signal transduction. These findings not only provide new insights into the cold response and acclimation of Arabidopsis, but also demonstrate the importance of studying changes in protein abundance within the relevant cellular compartment.
草本植物的冷驯化和越冬在能量方面代价高昂，且依赖于功能性质体代谢。为了解基质和类囊体腔蛋白质组如何适应低温，我们采用了蛋白质组学方法（差异凝胶电泳）来鉴定在冷休克（1天）以及在5摄氏度下短期（10天）和长期（40天）驯化过程中拟南芥叶绿体中丰度发生变化的蛋白质。我们发现，冷休克（1天）导致质体蛋白质组变化极小，而短期（10天）驯化导致基质蛋白质组发生重大变化，但类囊体腔蛋白质组变化很小。长期驯化（40天）导致两个区室的蛋白质组均发生调节，类囊体腔中出现新蛋白质，基质中蛋白质丰度进一步发生调节。我们鉴定出43种差异表达的蛋白质，它们参与光合作用、其他质体代谢功能、激素生物合成以及应激感知和信号转导。这些发现不仅为拟南芥的冷响应和驯化提供了新见解，还证明了研究相关细胞区室内蛋白质丰度变化的重要性。
