Cervelló I, Martínez-Conejero J A, Horcajadas J A, Pellicer A, Simón C
Fundación Instituto Valenciano de Infertilidad, Instituto Universitario IVI, Valencia University, Spain.
Hum Reprod. 2007 Jan;22(1):45-51. doi: 10.1093/humrep/del332. Epub 2006 Aug 21.
The endometrium, lining of the uterus, is a highly active organ that is remodelled periodically during the lifespan. Different studies suggest the presence of an adult or progenitor stem cell (PSC) population in this tissue because of its cyclic regenerative capacity.
In this study, we aim at identifying and localizing the putative PSC population in the murine uterus using the 5-bromo-2'-deoxyuridine (BrdU) labelling method to detect label-retaining cells (LRCs) that cycle slowly. Uteri from BrdU-treated mice were analysed via single and double immunohistochemistry to co-localize them with the markers of undifferentiation already described such as c-KIT and POU5F1 (also known as OCT-4). Finally, we confirmed the presence of the indicated markers at mRNA level.
We observed the presence and gradual decrease of LRCs in the endometrium during the lifespan of the mice. In adulthood, the LRC population decreased notably and remained in the lower region of the stroma in the murine endometrium. Some of the endometrial LRCs co-localized with c-KIT and POU5F1. PCR and nested-PCR confirmed the presence of these undifferentiated markers.
We demonstrated that the murine endometrium possesses LRCs with the features of a putative PSC population localized at the lower region of the stroma.
子宫内膜作为子宫的内衬，是一个高度活跃的器官，在整个生命周期中会周期性地重塑。由于其周期性再生能力，不同的研究表明该组织中存在成年干细胞或祖细胞（PSC）群体。
在本研究中，我们旨在使用5-溴-2'-脱氧尿苷（BrdU）标记法来鉴定和定位小鼠子宫中假定的PSC群体，以检测循环缓慢的标记保留细胞（LRC）。通过单免疫组化和双免疫组化分析经BrdU处理的小鼠的子宫，将LRC与已描述的未分化标记物如c-KIT和POU5F1（也称为OCT-4）共定位。最后，我们在mRNA水平上证实了这些标记物的存在。
我们观察到在小鼠的生命周期中，子宫内膜中存在LRC且其数量逐渐减少。在成年期，LRC群体显著减少，并保留在小鼠子宫内膜基质的下部区域。一些子宫内膜LRC与c-KIT和POU5F1共定位。PCR和巢式PCR证实了这些未分化标记物的存在。
我们证明了小鼠子宫内膜拥有具有假定PSC群体特征的LRC，其定位于基质的下部区域。
