大肠杆菌辅酶Q生物合成中泛醌G O-甲基转移酶与泛醌X相互作用的遗传证据。
Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis.
作者信息
Gulmezian Melissa, Zhang Haitao, Javor George T, Clarke Catherine F
机构信息
Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095-1569, USA.
出版信息
J Bacteriol. 2006 Sep;188(17):6435-9. doi: 10.1128/JB.00668-06.
Abstract
IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiX(K-12) reverses this phenotype, suggesting that UbiX may interact with UbiG.
摘要
IS16是一种对硫醇敏感、缺乏泛醌（Q）的大肠杆菌突变菌株。在此，我们表明IS16在编码泛醌生物合成两个O-甲基化步骤所需甲基转移酶的ubiG基因中存在突变。用ubiG或ubiX（K-12）对IS16进行互补可逆转此表型，这表明UbiX可能与UbiG相互作用。
相似文献
1
Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis.大肠杆菌辅酶Q生物合成中泛醌G O-甲基转移酶与泛醌X相互作用的遗传证据。
J Bacteriol. 2006 Sep;188(17):6435-9. doi: 10.1128/JB.00668-06.
2
Complementation of coq3 mutant yeast by mitochondrial targeting of the Escherichia coli UbiG polypeptide: evidence that UbiG catalyzes both O-methylation steps in ubiquinone biosynthesis.通过将大肠杆菌UbiG多肽定位于线粒体来互补coq3突变酵母：证明UbiG催化泛醌生物合成中的两个O-甲基化步骤。
Biochemistry. 1996 Jul 30;35(30):9797-806. doi: 10.1021/bi9602932.
3
The role of UbiX in Escherichia coli coenzyme Q biosynthesis.泛醌X在大肠杆菌辅酶Q生物合成中的作用。
Arch Biochem Biophys. 2007 Nov 15;467(2):144-53. doi: 10.1016/j.abb.2007.08.009. Epub 2007 Aug 23.
4
Restoring de novo coenzyme Q biosynthesis in Caenorhabditis elegans coq-3 mutants yields profound rescue compared to exogenous coenzyme Q supplementation.与外源辅酶 Q 补充相比，在 Caenorhabditis elegans coq-3 突变体中恢复从头辅酶 Q 生物合成可产生显著的挽救效果。
Gene. 2012 Sep 10;506(1):106-16. doi: 10.1016/j.gene.2012.06.023. Epub 2012 Jun 23.
5
Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis.酵母和大鼠的辅酶Q3（Coq3）以及大肠杆菌的泛醌合成酶UbiG多肽催化辅酶Q生物合成中的两个O-甲基转移酶步骤。
J Biol Chem. 1999 Jul 30;274(31):21665-72. doi: 10.1074/jbc.274.31.21665.
6
Regulation of the isofunctional genes ubiD and ubiX of the ubiquinone biosynthetic pathway of Escherichia coli.大肠杆菌泛醌生物合成途径中同功基因ubiD和ubiX的调控
FEMS Microbiol Lett. 2003 Jun 6;223(1):67-72. doi: 10.1016/S0378-1097(03)00343-4.
7
Low ubiquinone content in Escherichia coli causes thiol hypersensitivity.大肠杆菌中泛醌含量低会导致硫醇超敏反应。
J Bacteriol. 1998 Jul;180(14):3681-5. doi: 10.1128/JB.180.14.3681-3685.1998.
8
Isolation and characterization of Escherichia coli mutants affected in aerobic respiration: the cloning and nucleotide sequence of ubiG. Identification of an S-adenosylmethionine-binding motif in protein, RNA, and small-molecule methyltransferases.有氧呼吸受影响的大肠杆菌突变体的分离与特性分析：泛醌合成基因ubiG的克隆及核苷酸序列。蛋白质、RNA和小分子甲基转移酶中S-腺苷甲硫氨酸结合基序的鉴定。
J Gen Microbiol. 1992 Oct;138(10):2101-12. doi: 10.1099/00221287-138-10-2101.
9
10
Identification of a ubiG-like gene involved in ubiquinone biosynthesis from Chlamydophila pneumoniae AR39.从肺炎衣原体AR39中鉴定出一个参与泛醌生物合成的类ubiG基因。
Lett Appl Microbiol. 2007 Jul;45(1):47-54. doi: 10.1111/j.1472-765X.2007.02144.x.
引用本文的文献
1
Biosynthesis of Menaquinone (Vitamin K2) and Ubiquinone (Coenzyme Q).甲萘醌（维生素K2）和泛醌（辅酶Q）的生物合成
EcoSal Plus. 2009 Aug;3(2). doi: 10.1128/ecosalplus.3.6.3.3.
2
Chorismate pyruvate-lyase and 4-hydroxy-3-solanesylbenzoate decarboxylase are required for plastoquinone biosynthesis in the cyanobacterium Synechocystis sp. PCC6803.分支酸丙酮酸裂解酶和 4-羟基-3-茄呢醇基苯甲酸脱羧酶是蓝藻集胞藻 PCC6803 质体醌生物合成所必需的。
J Biol Chem. 2014 Jan 31;289(5):2675-86. doi: 10.1074/jbc.M113.511709. Epub 2013 Dec 11.
3
Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli.来自大肠杆菌的O-甲基转移酶UbiG的结晶及初步晶体学研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jun 1;67(Pt 6):727-9. doi: 10.1107/S1744309111014278. Epub 2011 May 26.
4
The role of UbiX in Escherichia coli coenzyme Q biosynthesis.泛醌X在大肠杆菌辅酶Q生物合成中的作用。
Arch Biochem Biophys. 2007 Nov 15;467(2):144-53. doi: 10.1016/j.abb.2007.08.009. Epub 2007 Aug 23.
本文引用的文献
1
Uncoupling the pleiotropic phenotypes of clk-1 with tRNA missense suppressors in Caenorhabditis elegans.利用秀丽隐杆线虫中的tRNA错义抑制因子解开clk-1的多效性表型
Mol Cell Biol. 2006 May;26(10):3976-85. doi: 10.1128/MCB.26.10.3976-3985.2006.
2
Complementation of Saccharomyces cerevisiae coq7 mutants by mitochondrial targeting of the Escherichia coli UbiF polypeptide: two functions of yeast Coq7 polypeptide in coenzyme Q biosynthesis.通过将大肠杆菌UbiF多肽靶向线粒体来互补酿酒酵母coq7突变体：酵母Coq7多肽在辅酶Q生物合成中的两种功能
J Biol Chem. 2006 Jun 16;281(24):16401-9. doi: 10.1074/jbc.M513267200. Epub 2006 Apr 19.
3
Biosynthesis, bioproduction and novel roles of ubiquinone.泛醌的生物合成、生物生产及新作用
J Biosci Bioeng. 2002;94(6):511-7. doi: 10.1016/s1389-1723(02)80188-8.
4
COQ9, a new gene required for the biosynthesis of coenzyme Q in Saccharomyces cerevisiae.COQ9，酿酒酵母中辅酶Q生物合成所需的一个新基因。
J Biol Chem. 2005 Sep 9;280(36):31397-404. doi: 10.1074/jbc.M503277200. Epub 2005 Jul 18.
5
fldA is an essential gene required in the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis.fldA是2-C-甲基-D-赤藓糖醇-4-磷酸途径中类异戊二烯生物合成所需的一个必需基因。
FEBS Lett. 2005 Jul 4;579(17):3802-6. doi: 10.1016/j.febslet.2005.05.047.
6
Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q.Coq3和Coq4在酵母线粒体中定义了一种用于辅酶Q生物合成的多肽复合物。
J Biol Chem. 2005 May 27;280(21):20231-8. doi: 10.1074/jbc.M501315200. Epub 2005 Mar 25.
7
Interaction network containing conserved and essential protein complexes in Escherichia coli.包含大肠杆菌中保守且必需蛋白质复合物的相互作用网络。
Nature. 2005 Feb 3;433(7025):531-7. doi: 10.1038/nature03239.
8
Genetic evidence for a multi-subunit complex in coenzyme Q biosynthesis in yeast and the role of the Coq1 hexaprenyl diphosphate synthase.酵母辅酶Q生物合成中多亚基复合物的遗传证据以及Coq1十六异戊二烯基二磷酸合酶的作用。
J Biol Chem. 2005 Jan 28;280(4):2676-81. doi: 10.1074/jbc.M411527200. Epub 2004 Nov 17.
9
Yeast Coq5 C-methyltransferase is required for stability of other polypeptides involved in coenzyme Q biosynthesis.酵母辅酶Q5 C-甲基转移酶是辅酶Q生物合成中其他参与多肽稳定性所必需的。
J Biol Chem. 2004 Mar 12;279(11):10052-9. doi: 10.1074/jbc.M313712200. Epub 2003 Dec 29.
10
Automated identification of putative methyltransferases from genomic open reading frames.从基因组开放阅读框中自动识别假定的甲基转移酶。
Mol Cell Proteomics. 2003 Aug;2(8):525-40. doi: 10.1074/mcp.M300037-MCP200. Epub 2003 Jul 18.
Zotero 插件