Sufian M K N B, Hira Tohru, Miyashita Kyoko, Nishi Takashi, Asano Kozo, Hara Hiroshi
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Biosci Biotechnol Biochem. 2006 Aug;70(8):1869-74. doi: 10.1271/bbb.60046.
We found that soybean beta-conglycinin peptone (BconP) suppresses food intake through cholecystokinin (CCK) release from enteroendocrine cells in association with binding of the peptone to rat small intestinal brush border membrane (BBM). The aim of the present study was to find new appetite suppressing peptides. Peptones from chicken, pork, beef, beef liver, and egg white were examined for activities to bind with rat BBM, CCK-release from enteroendocrine cell line STC-1, and induce satiety in rats. Chicken and pork peptone (ChickP and PorkP) bound to BBM with highest ability as evaluated with a surface plasmon biosensor. PorkP and ChickP released CCK in higher amounts than BconP from STC-1 cells dose-dependently, with highest stimulation by PorkP. An orogastric preload of PorkP, but not ChickP, suppressed food intake similarly to BconP, dose-dependently. These results suggest that PorkP interacts directly with the small intestinal CCK cells to release CCK, and that it suppresses appetite in rats.
我们发现大豆β-伴大豆球蛋白蛋白胨（BconP）通过肠内分泌细胞释放胆囊收缩素（CCK）来抑制食物摄入，这与该蛋白胨与大鼠小肠刷状缘膜（BBM）的结合有关。本研究的目的是寻找新的食欲抑制肽。检测了来自鸡肉、猪肉、牛肉、牛肝和蛋清的蛋白胨与大鼠BBM的结合活性、从肠内分泌细胞系STC-1释放CCK的活性以及在大鼠中诱导饱腹感的活性。用表面等离子体生物传感器评估发现，鸡肉和猪肉蛋白胨（ChickP和PorkP）与BBM的结合能力最强。PorkP和ChickP从STC-1细胞中剂量依赖性地释放出比BconP更多的CCK，其中PorkP的刺激作用最强。经口胃预负荷PorkP（而非ChickP）与BconP类似，能剂量依赖性地抑制食物摄入。这些结果表明，PorkP直接与小肠CCK细胞相互作用以释放CCK，并能抑制大鼠的食欲。
