Dhonukshe Pankaj, Bargmann Bastiaan O R, Gadella Theodorus W J
Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands.
Plant Cell Physiol. 2006 Oct;47(10):1406-11. doi: 10.1093/pcp/pcl001. Epub 2006 Aug 22.
Microtubule biogenesis requires alphabeta tubulin dimers that are generated from alpha and beta tubulin following post-translational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The subcellular localization of AtTFCB is mainly cytosolic. AtTFCB-overexpressing cells have fewer microtubules compared with the controls. Multimode fluorescence resonance energy transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with alpha tubulin in living plant cells. We conclude that AtTFCB interacts with alpha tubulin in vivo and its overexpression reduces the number of microtubules.
微管生物发生需要αβ微管蛋白二聚体，这些二聚体是由α和β微管蛋白在经过几种微管蛋白折叠辅助因子（TFCs）的翻译后修饰后产生的。在此，我们报告了拟南芥TFCB（AtTFCB）的分离和特性。AtTFCB在拟南芥的所有器官中均有表达。AtTFCB的亚细胞定位主要在细胞质中。与对照相比，过表达AtTFCB的细胞中的微管较少。多模荧光共振能量转移（FRET）显微镜显示AtTFCB与活植物细胞中的α微管蛋白存在直接的物理相互作用。我们得出结论，AtTFCB在体内与α微管蛋白相互作用，并且其过表达会减少微管的数量。
