Kaye M, Hodsman A B, Malynowsky L
Division of Nephrology, Montreal General Hospital, Quebec, Canada.
Kidney Int. 1990 Apr;37(4):1142-7. doi: 10.1038/ki.1990.97.
Fourteen individuals on long term hemodialysis, with varying amounts of aluminum in bone from 11 to 296 mg/kg by flameless atomic absorption spectrophotometry, were examined to see whether the aluminon or acid solochrome azurine (ASA) staining reactions best approximated the chemical determination. Correlation coefficients were 0.78 for aluminon and 0.88 for ASA. Together with 11 additional patients morphometric parameters were compared with the two aluminum stains. The aluminon stain gave satisfactory results in the osteomalacic group but underestimated the amount of aluminum present in those with hyperparathyroid, mixed or aplastic disease. Some individuals showed a striking difference between the two techniques which could have led to an erroneous conclusion regarding the amount of aluminum present. The aluminon stain was pH dependent and together with ASA could be enhanced by prior heat treatment of the sections. It is recommended that ASA either replace aluminon for routine use or be used together with the aluminon stain, particularly for bones without osteomalacia or with mild to moderate aluminum storage.
对14名长期接受血液透析的患者进行了检查，通过无火焰原子吸收分光光度法测定其骨中铝含量在11至296毫克/千克之间，以确定铝试剂或酸性铬蓝素（ASA）染色反应是否最接近化学测定结果。铝试剂的相关系数为0.78，ASA的相关系数为0.88。连同另外11名患者，将形态学参数与两种铝染色法进行了比较。铝试剂染色在骨软化症组中给出了满意的结果，但低估了甲状旁腺功能亢进、混合性或再生障碍性疾病患者体内的铝含量。一些个体在两种技术之间显示出显著差异，这可能导致关于体内铝含量的错误结论。铝试剂染色依赖于pH值，并且与ASA一起可以通过对切片进行预先热处理来增强。建议ASA要么取代铝试剂用于常规使用，要么与铝试剂染色一起使用，特别是对于没有骨软化症或铝储存轻度至中度的骨骼。
