Colbert Costa M
Department of Biology and Biochemistry, University of Houston, TX, USA.
Methods Mol Biol. 2006;337:117-25. doi: 10.1385/1-59745-095-2:117.
Acute brain slices allow electrophysiological and imaging techniques to be applied in vitro to the study of neuronal ion channels, synaptic plasticity, and whole-cell function in juvenile and adult tissue. Ion channel recordings from small dendritic branches and axons in brain slices have demonstrated considerable functional differences in ion channel function within subregions of single cells. These findings have greatly increased our understanding of neuronal computation. This chapter presents methods for obtaining high-quality brain slices developed to aid visualization of small neuronal structures using differential interference contrast microscopy.
急性脑片使电生理和成像技术能够在体外应用于研究幼年和成年组织中的神经元离子通道、突触可塑性及全细胞功能。对脑片中小的树突分支和轴突进行离子通道记录，已证实在单个细胞的亚区域内离子通道功能存在显著的功能差异。这些发现极大地增进了我们对神经元计算的理解。本章介绍了获取高质量脑片的方法，这些方法旨在利用微分干涉对比显微镜辅助观察小的神经元结构。
