Hendrickson Luke, Vlcková Alexandra, Selstam Eva, Huner Norman, Oquist Gunnar, Hurry Vaughan
Umeå Plant Science Centre, Department of Plant Physiology, University of Umeå, Umeå S-901 87, Sweden.
FEBS Lett. 2006 Sep 4;580(20):4959-68. doi: 10.1016/j.febslet.2006.07.081. Epub 2006 Aug 8.
We compared the thylakoid membrane composition and photosynthetic properties of non- and cold-acclimated leaves from the dgd1 mutant (lacking >90% of digalactosyl-diacylglycerol; DGDG) and wild type (WT) Arabidopsis thaliana. In contrast to warm grown plants, cold-acclimated dgd1 leaves recovered pigment-protein pools and photosynthetic function equivalent to WT. Surprisingly, this recovery was not correlated with an increase in DGDG. When returned to warm temperatures the severe dgd1 mutant phenotype reappeared. We conclude that the relative recovery of photosynthetic activity at 5 degrees C resulted from a temperature/lipid interaction enabling the stable assembly of PSI complexes in the thylakoid.
我们比较了双半乳糖二酰基甘油缺乏突变体（dgd1，缺乏>90%的双半乳糖二酰基甘油；DGDG）和野生型拟南芥在未冷驯化和冷驯化叶片中的类囊体膜组成及光合特性。与温暖环境下生长的植株不同，冷驯化的dgd1叶片恢复了与野生型相当的色素-蛋白库和光合功能。令人惊讶的是，这种恢复与DGDG的增加并无关联。当回到温暖温度时，严重的dgd1突变体表型再次出现。我们得出结论，5摄氏度时光合活性的相对恢复是由于温度/脂质相互作用使得PSI复合体能够在类囊体中稳定组装。
