Buehring G C
School of Public Health, University of California, Berkeley 94720.
J Dairy Sci. 1990 Apr;73(4):956-63. doi: 10.3168/jds.S0022-0302(90)78752-8.
Mammary epithelial cells are exfoliated into cows' milk and comprise a component of the somatic cells enumerated for milk quality control. This paper described the finding that these epithelial cells can be recovered from milk and grown in culture. The cultured cells were characterized for growth rate and growth potential, morphology by light and electron microscopy, presence of esterases and cytokeratins, and sensitivity to storage at 4 degrees C. Cultured mammary epithelial cells from milk may be useful to dairy scientists and mammary gland biologists.
乳腺上皮细胞会脱落到牛奶中，并且是用于牛奶质量控制而计数的体细胞的组成部分。本文描述了从牛奶中回收这些上皮细胞并在培养中生长的这一发现。对培养的细胞进行了生长速率和生长潜力、光镜和电镜下的形态、酯酶和细胞角蛋白的存在情况以及对4℃储存的敏感性等方面的表征。从牛奶中培养的乳腺上皮细胞可能对乳品科学家和乳腺生物学家有用。
