Last J A, Gerriets J E, Armstrong L C, Gelzleichter T R, Reiser K M
Department of Internal Medicine, School of Medicine, University of California, Davis.
Am J Respir Cell Mol Biol. 1990 Jun;2(6):543-8. doi: 10.1165/ajrcmb/2.6.543.
Collagen synthesized by tissue minces from lungs of rats administered 1 unit of bleomycin by intratracheal instillation 1 or 2 wk earlier contained relatively more hydroxylysine than did collagen made by lungs from saline-instilled control animals. Most, if not all, of the relative increase in lysine hydroxylation could be localized to the alpha 1 (I) chain of type I collagen. Lung homogenates from bleomycin-treated rats showed increased activity of lysyl hydroxylase (EC 1.14.11.4), the enzyme catalyzing the conversion of collagen-bound lysine to hydroxylysine. Thus, the increased hydroxylation of lysine and of lysine-derived cross-links previously observed in collagen of diseased human lungs and in animal models of lung fibrosis is reflected in an in vitro system.
通过气管内滴注给予1单位博来霉素1或2周前的大鼠肺组织碎片合成的胶原蛋白，与用生理盐水滴注的对照动物的肺组织合成的胶原蛋白相比，含有相对更多的羟赖氨酸。赖氨酸羟化的相对增加，即使不是全部，大部分也可定位于I型胶原蛋白的α1（I）链。博来霉素处理的大鼠的肺匀浆显示赖氨酰羟化酶（EC 1.14.11.4）的活性增加，该酶催化胶原蛋白结合的赖氨酸转化为羟赖氨酸。因此，先前在患病人类肺组织和肺纤维化动物模型的胶原蛋白中观察到的赖氨酸和赖氨酸衍生交联的羟化增加在体外系统中得到了体现。
