Chen Yanke, Zhao Zhongliang, Liu Naifa
School of Life Science, Lanzhou University, Lanzhou, 730000, P.R. China,
Biochem Genet. 2006 Jun;44(5-6):209-21. doi: 10.1007/s10528-006-9024-3. Epub 2006 Aug 25.
In this paper, we have analyzed the nuclear DNA of Alectoris magna samples, collected from the Longzhong Loess Plateau. We used allelic variation at eight microsatellite markers to describe the genetic structure of A. magna populations. The primary goal of this study was to examine the population genetic structure and determine the extent of population differentiation among populations of A. magna. The average value of H(E) (0.455) was smaller than H(O) (0.477), and there was a heterozygote deficit at the MCW135 locus in the Lanzhou population and the Beidao population. The AB063167 locus in each population (except that of Jingyuan) was not in equilibrium (P < 0.05). The Lixian edge population had a lower proportion of genetic diversity than the central geographic populations; the Haiyuan and Jingyuan populations had higher genetic diversity than the central geographic populations. Analysis of population structure revealed clear differentiation among the eight populations of A. magna, suggesting strong isolation of these populations and correspondingly low levels of migration or gene flow. The A. magna populations of Longzhong Loess Plateau are separated into eastern and western populations by a clustering chart. Genetic data indeed suggest that patterns of speciation and population diversification of A. magna in the Longzhong Plateau have been affected by the stability of the climate, natural selection, and human intervention.
在本文中，我们分析了采自陇中黄土高原的大石鸡样本的核DNA。我们使用八个微卫星标记的等位基因变异来描述大石鸡种群的遗传结构。本研究的主要目的是研究大石鸡种群的遗传结构，并确定大石鸡种群之间的种群分化程度。H(E)的平均值（0.455）小于H(O)（0.477），并且兰州种群和北道种群的MCW135位点存在杂合子缺失。每个种群（靖远种群除外）的AB063167位点均不平衡（P < 0.05）。礼县边缘种群的遗传多样性比例低于中部地理种群；海原种群和靖远种群的遗传多样性高于中部地理种群。种群结构分析显示，大石鸡的八个种群之间存在明显分化，表明这些种群之间存在强烈隔离，相应地迁移或基因流水平较低。通过聚类图，陇中黄土高原的大石鸡种群被分为东部种群和西部种群。遗传数据确实表明，陇中高原大石鸡的物种形成和种群多样化模式受到气候稳定性、自然选择和人类干预的影响。
