Kundra V, Dean M F
Kennedy Institute of Rheumatology, Hammersmith, London, United Kingdom.
Exp Cell Res. 1990 Jul;189(1):93-9. doi: 10.1016/0014-4827(90)90261-8.
The secreted and intracellular activities of a number of lysosomal hydrolases were higher in 3T3 cells than in SV40-transformed cells. The number of lysosomes and their total volume were also much larger in 3T3 cells and the surface area of their lysosomal membranes was almost twice that of SV3T3 cells. These differences alone were not sufficiently large, however, to account for the disparity seen in activity of some enzymes. Gel electrophoresis showed that a number of protein components present in lysosomal membranes purified from 3T3 cells were absent from SV3T3 membrane preparations. The absence of these components may be correlated with the reduced enzyme activity of SV3T3 cells particularly with respect to beta-glucosidase and acid phosphatase, both of which are normally found associated with lysosomal membranes.
许多溶酶体水解酶的分泌活性和细胞内活性在3T3细胞中比在SV40转化细胞中更高。3T3细胞中的溶酶体数量及其总体积也大得多，其溶酶体膜的表面积几乎是SV3T3细胞的两倍。然而，仅这些差异还不够大，不足以解释某些酶活性中观察到的差异。凝胶电泳显示，从3T3细胞纯化的溶酶体膜中存在的许多蛋白质成分在SV3T3膜制剂中不存在。这些成分的缺失可能与SV3T3细胞酶活性降低有关，特别是对于β-葡萄糖苷酶和酸性磷酸酶，这两种酶通常与溶酶体膜相关。
