一个编码对丙型肝炎感染具有高度特异性的肽的cDNA克隆。

A cDNA clone encoding a peptide highly specific for hepatitis C infection.

作者信息

Arima T, Mori C, Takamizawa A, Shimomura H, Tsuji T

机构信息

First Department of Internal Medicine, Okayama University Medical School, Japan.

出版信息

Gastroenterol Jpn. 1990 Apr;25(2):218-22. doi: 10.1007/BF02776819.

DOI:10.1007/BF02776819

PMID:1693349

Abstract

A random primed lambda gt11-cDNA library was constructed from donors plasma presumably infected by blood-borne non-A, non-B hepatitis (hepatitis C:HC) agent and immunoscreened with serum pooled from patients with acute or chronic HC. Twelve lambda gt11-cDNA clones encoding antigens associated with HC infection in Japan as well as in the USA were isolated. Of these one clone consisting of 114 nucleotides and showing a discrete band on an immunoblot analysis, was extensively studied. The clone is not derived from the host DNA encoding one polypeptide specific and highly sensitive for serum from patients with HC and has no homology to the nucleotide sequences of known human viruses including hepatitis A,B and D viruses, Ebstein-Barr virus, coxsackievirus, immunodeficiency virus type 1 or Japanese encephalitis virus. These results suggest that this clone is derived from the genome of HC agent.

摘要

用可能感染了血源性非甲非乙型肝炎（丙型肝炎：HC）病原体的供血者血浆构建了一个随机引物λgt11-cDNA文库，并用急性或慢性HC患者的混合血清进行免疫筛选。分离出了12个编码与日本和美国HC感染相关抗原的λgt11-cDNA克隆。其中一个由114个核苷酸组成、在免疫印迹分析中显示出一条离散条带的克隆被广泛研究。该克隆并非来自编码对HC患者血清具有特异性且高度敏感的一种多肽的宿主DNA，并且与已知人类病毒（包括甲型、乙型和丁型肝炎病毒、爱泼斯坦-巴尔病毒、柯萨奇病毒、1型免疫缺陷病毒或日本脑炎病毒）的核苷酸序列无同源性。这些结果表明该克隆源自HC病原体的基因组。