针对大麦α-淀粉酶I的单克隆抗体：制备、特性鉴定及其在特定免疫测定中的应用。

Monoclonal antibodies to barley alpha-amylase I: production, characterization, and application to a specific immunoassay.

作者信息

Lecommandeur D, Merlin F, Daussant J, Van Hoegaerden M

机构信息

Laboratoire de Physiologie des Organes Végétaux, C.N.R.S., Meudon, France.

出版信息

Hybridoma. 1990 Apr;9(2):177-87. doi: 10.1089/hyb.1990.9.177.

DOI:10.1089/hyb.1990.9.177

PMID:1693360

Abstract

Monoclonal antibodies (MAbs) to barley alpha-amylase I have been produced, purified and characterized. Six are specific for the alpha-amylase I isoform, while one also reacts with alpha-amylase II. All but one recognize the antigen in solution. Topology of the MAbs was investigated by additivity in ELISA, which led us to subdivide them into four groups. An immunoassay was developed using one of these MAbs, to measure alpha-amylase I activity in an extract containing both isoforms.

摘要

已制备、纯化并鉴定了针对大麦α-淀粉酶I的单克隆抗体（MAb）。其中六种对α-淀粉酶I同工型具有特异性，而有一种还与α-淀粉酶II发生反应。除一种外，其余所有抗体均能识别溶液中的抗原。通过酶联免疫吸附测定（ELISA）中的加和性研究了单克隆抗体的拓扑结构，这使我们将它们分为四组。使用其中一种单克隆抗体制备了一种免疫测定法，用于测量含有两种同工型的提取物中的α-淀粉酶I活性。