Qadri A, Ghosh S, Prakash K, Kumar R, Moudgil K D, Talwar G P
National Institute of Immunology, New Delhi.
J Immunoassay. 1990;11(2):251-69. doi: 10.1080/01971529008053272.
Enzyme immunoassays were developed using monoclonal antibodies raised against somatic (O), flagellar (H) and capsular (Vi) antigens of Salmonella typhi. The assay based on anti-O monoclonal antibodies could specifically detect S. typhi and soluble lipopolysaccharide (LPS) isolated from S. typhi. Anti-H MoAbs detected motile S. typhi and soluble flagellar antigen. Monoclonal antibodies against capsular polysaccharide could detect Vi-containing S. typhi as well as soluble Vi antigen. The three assays reported here detected S. typhi with 100% sensitivity in blood culture broths obtained from bacteriologically confirmed typhoid patients and were negative with blood specimens containing Salmonella senftenberg, E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis or Streptococcus (alpha-hemolytic) derived from patients with pyrexia. The assays, however, did not demonstrate the presence of soluble antigens in sera and urine samples obtained from typhoid patients.
利用针对伤寒沙门氏菌菌体（O）、鞭毛（H）和荚膜（Vi）抗原产生的单克隆抗体开发了酶免疫测定法。基于抗O单克隆抗体的测定法能够特异性检测伤寒沙门氏菌以及从伤寒沙门氏菌中分离出的可溶性脂多糖（LPS）。抗H单克隆抗体可检测运动性伤寒沙门氏菌和可溶性鞭毛抗原。针对荚膜多糖的单克隆抗体能够检测含Vi的伤寒沙门氏菌以及可溶性Vi抗原。本文报道的这三种测定法在从经细菌学确诊的伤寒患者获得的血培养肉汤中检测伤寒沙门氏菌的灵敏度为100%，而对来自发热患者的含有肠炎沙门氏菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、奇异变形杆菌或链球菌（α溶血）的血标本呈阴性。然而，这些测定法并未证明在从伤寒患者获得的血清和尿液样本中存在可溶性抗原。
