Roelofs J B, Graat E A M, Mullaart E, Soede N M, Voskamp-Harkema W, Kemp B
Adaptation Physiology Group, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands.
Theriogenology. 2006 Dec;66(9):2173-81. doi: 10.1016/j.theriogenology.2006.07.005. Epub 2006 Aug 28.
The objective of this study was to examine effects of the interval between insemination and ovulation on fertilization and embryo characteristics (quality scored as good, fair, poor and degenerate; morphology; number of cell cycles and accessory sperm number) in dairy cattle. Time of ovulation was assessed by ultrasonography (every 4h). Cows were artificially inseminated once between 36h before ovulation and 12h after ovulation. In total 122 oocytes/embryos were recovered 7d after ovulation. Insemination-ovulation interval (12h-intervals) affected fertilization and the percentages of good embryos. Fertilization rates were higher when AI was performed between 36-24 and 24-12h before ovulation (85% and 82%) compared to AI after ovulation (56%). AI between 24 and 12h before ovulation resulted in higher percentages of good embryos (68%) compared to AI after ovulation (6%). Insemination-ovulation interval had no effect on number of accessory sperm cells and number of cell cycles when corrected for embryo quality. This study showed that the insemination-ovulation interval with a high probability of fertilization is quite long (from 36 to 12h before ovulation). However, the insemination-ovulation interval in which this fertilized oocyte has a high probability of developing into a good embryo is shorter (24-12h before ovulation).
本研究的目的是检测授精与排卵间隔时间对奶牛受精及胚胎特征（质量分为优、良、差和退化；形态；细胞周期数和附属精子数）的影响。通过超声检查（每4小时一次）评估排卵时间。在排卵前36小时至排卵后12小时之间对奶牛进行一次人工授精。排卵7天后共回收122个卵母细胞/胚胎。授精-排卵间隔时间（以12小时为间隔）影响受精及优质胚胎的比例。与排卵后授精（56%）相比，在排卵前36-24小时和24-12小时进行人工授精时受精率更高（分别为85%和82%）。与排卵后授精（6%）相比，在排卵前24-12小时进行人工授精产生的优质胚胎比例更高（68%）。校正胚胎质量后，授精-排卵间隔时间对附属精子细胞数和细胞周期数没有影响。本研究表明，具有较高受精概率的授精-排卵间隔时间相当长（从排卵前36小时至12小时）。然而，已受精的卵母细胞有较高概率发育成优质胚胎的授精-排卵间隔时间较短（排卵前24-12小时）。
