Fujita N, Ishiguro H, Sato S, Kurihara T, Kuwano R, Sakimura K, Takahashi Y, Miyatake T
Department of Neurology, Niigata University, Japan.
Brain Res. 1990 Apr 9;513(1):152-5. doi: 10.1016/0006-8993(90)91102-m.
Two forms of myelin-associated glycoprotein (MAG) mRNA are produced by alternative splicing of the exon 12 portion. Expression of the two forms of MAG mRNA was studied here in experimentally introduced demyelination and remyelination by Cuprizone intoxication. During the demyelinating stage, both forms of MAG mRNA decreased markedly. When feeding with Cuprizone was stopped, MAG mRNA began to increase. One form of MAG mRNA without the exon 12 portion, which appears in normal development at the period of active myelination, was characteristically induced during the remyelinating stage. The other form containing the exon 12 portion was also induced but recovered only to the level in normal development.
髓鞘相关糖蛋白（MAG）的两种mRNA形式是通过外显子12部分的可变剪接产生的。本文研究了通过铜离子螯合剂中毒实验诱导的脱髓鞘和髓鞘再生过程中两种MAG mRNA形式的表达。在脱髓鞘阶段，两种MAG mRNA形式均显著下降。停止喂食铜离子螯合剂后，MAG mRNA开始增加。在髓鞘再生阶段，一种不含外显子12部分的MAG mRNA形式被特异性诱导，这种形式在正常发育的活跃髓鞘形成期出现。另一种含有外显子12部分的形式也被诱导，但仅恢复到正常发育水平。
