用于同时定量测定人血浆中游离霉酚酸及其葡萄糖醛酸代谢物的简单反相液相色谱分析法。
Simple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma.
作者信息
Yau Wai-Ping, Vathsala Anantharaman, Lou Huei-Xin, Zhou Shu Feng, Chan Eli
机构信息
Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 1;846(1-2):313-8. doi: 10.1016/j.jchromb.2006.07.062. Epub 2006 Aug 28.
A reversed-phase HPLC-UV method, involving simple instrumental setup and mobile phase without ion-pairing reagent, was developed and validated for direct simultaneous quantification of free mycophenolic acid (MPA) and its major metabolite MPA-glucuronide (MPAG) in human plasma. Both free MPA and MPAG were isolated from plasma samples using ultrafiltration prior to analysis. Each chromatographic run was completed within 13 min. The optimized method showed good performance in terms of specificity, linearity (r(2)=0.9999), sensitivity (limit of quantitation (LOQ): 0.005 mg/L for MPA; 1 mg/L for MPAG), and intra- and inter-day precision (R.S.D.<7%). This assay was successfully applied to free MPA and MPAG measurements in clinical samples.
建立了一种反相高效液相色谱 - 紫外检测法，该方法仪器设置简单，流动相无需离子对试剂，可直接同时定量测定人血浆中游离霉酚酸（MPA）及其主要代谢物MPA - 葡萄糖醛酸苷（MPAG）。分析前，采用超滤法从血浆样品中分离出游离MPA和MPAG。每次色谱运行在13分钟内完成。优化后的方法在特异性、线性（r(2)=0.9999）、灵敏度（定量限（LOQ）：MPA为0.005 mg/L；MPAG为1 mg/L）以及日内和日间精密度（相对标准偏差<7%）方面表现良好。该方法已成功应用于临床样品中游离MPA和MPAG的测定。
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