Yau Wai-Ping, Vathsala Anantharaman, Lou Huei-Xin, Chan Eli
Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 5;805(1):101-12. doi: 10.1016/j.jchromb.2004.02.020.
A simple and reproducible reversed-phase ion-pair high-performance liquid chromatographic (HPLC) method using isocratic elution with UV absorbance detection is presented for the simultaneous quantitation of mycophenolic acid (MPA) and MPA-glucuronide (MPAG) in human plasma and urine. The sample preparation procedures involved simple protein precipitation for plasma and 10-fold dilution for urine. Each analytical run was completed within 15min, with MPAG and MPA being eluted at 3.8 and 11.4min, respectively. The optimized method showed good performance in terms of specificity, linearity, detection and quantitation limits, precision and accuracy. This assay was demonstrated to be applicable for clinical pharmacokinetic studies.
本文提出了一种简单且可重复的反相离子对高效液相色谱（HPLC）方法，采用等度洗脱和紫外吸收检测，用于同时定量测定人血浆和尿液中的霉酚酸（MPA）和MPA-葡萄糖醛酸苷（MPAG）。样品制备程序包括血浆的简单蛋白沉淀和尿液的10倍稀释。每次分析运行在15分钟内完成，MPAG和MPA分别在3.8分钟和11.4分钟洗脱。优化后的方法在特异性、线性、检测和定量限、精密度和准确度方面表现良好。该测定法被证明适用于临床药代动力学研究。
