Chang Pei-Yao, Carrel Hyman, Huang Jen-Shang, Wang I-Jong, Hou Yu-Chih, Chen Wei-Li, Wang Jiun-Yi, Hu Fung-Rong
Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
Am J Ophthalmol. 2006 Sep;142(3):488-90. doi: 10.1016/j.ajo.2006.04.033.
To define alterations in the density of corneal basal epithelium in relation to diabetic retinopathic severity and the alteration of corneal innervation using confocal microscopy.
Prospective case-control study.
Forty-two type II diabetic patients stratified into nondiabetic (NDR), nonproliferative diabetic (NPDR), and proliferative diabetic (PDR) retinopathy and 14 age-matched healthy control subjects were studied. Epithelial and stromal cell densities and subbasal nerves were investigated by confocal microscopy.
Corneal basal epithelial cell density (P = .0001), nerve fiber density (NFD) (P < .0001), nerve branch density (NBD) (P = .0003), and tortuosity coefficient (Tc) (P < .0001) were significantly different among the four groups. Basal epithelial density was significantly correlated with NFD (r = 0.43, P = .0009), NBD (r = 0.36, P = .007), and TC (r = -0.58, P < .0001).
Patients with diabetes show alterations in corneal innervations and basal epithelial cell density in different retinopathic stages. We demonstrate that reduced density in basal epithelial cell is correlated with changes in innervation.
利用共聚焦显微镜确定角膜基底上皮密度与糖尿病视网膜病变严重程度的关系以及角膜神经支配的改变。
前瞻性病例对照研究。
对42例II型糖尿病患者进行研究，这些患者被分为非糖尿病性（NDR）、非增殖性糖尿病性（NPDR）和增殖性糖尿病性（PDR）视网膜病变组，另有14名年龄匹配的健康对照受试者。通过共聚焦显微镜研究上皮和基质细胞密度以及基底膜下神经。
四组之间角膜基底上皮细胞密度（P = 0.0001）、神经纤维密度（NFD）（P < 0.0001）、神经分支密度（NBD）（P = 0.0003）和曲折系数（Tc）（P < 0.0001）存在显著差异。基底上皮密度与NFD（r = 0.43，P = 0.0009）、NBD（r = 0.36，P = 0.007）和TC（r = -0.58，P < 0.0001）显著相关。
糖尿病患者在不同视网膜病变阶段角膜神经支配和基底上皮细胞密度存在改变。我们证明基底上皮细胞密度降低与神经支配变化相关。
