Harper J D, Rao P N, John P C
Research School of Biological Sciences, Australian National University, Canberra.
Eur J Cell Biol. 1990 Apr;51(2):272-8.
The monoclonal antibody MPM-2 recognizes a family of phosphorylated proteins present in mitotic cells. In a number of organisms it stains nuclei and also cytoskeletal structures which contain or organize tubulin. In mitotic Chlamydomonas reinhardtii cells MPM-2 reacts with phosphoproteins associated with the nuclear envelope (NE). Staining of the NE region appears in preprophase, reaches a maximum intensity in metaphase/anaphase and disappears rapidly in telophase. Localized hyperphosphorylation of the anterior NE region is apparent in many cells throughout mitosis. The distribution and timing of MPM-2 labeling suggests that in Chlamydomonas MPM-2 may be interacting with lamin-like phosphoproteins.
单克隆抗体MPM-2可识别存在于有丝分裂细胞中的一类磷酸化蛋白。在许多生物体中，它可对细胞核以及含有或组织微管蛋白的细胞骨架结构进行染色。在有丝分裂的莱茵衣藻细胞中，MPM-2与核膜（NE）相关的磷蛋白发生反应。NE区域的染色在前期开始出现，在中期/后期达到最大强度，并在末期迅速消失。在整个有丝分裂过程中，许多细胞的前NE区域都出现局部过度磷酸化。MPM-2标记的分布和时间表明，在衣藻中MPM-2可能与核纤层样磷蛋白相互作用。
