Mackay A M, Tracy R P, Craighead J E
Department of Pathology, University of Vermont, College of Medicine, Burlington 05405.
J Cell Sci. 1990 Jan;95 ( Pt 1):97-107. doi: 10.1242/jcs.95.1.97.
Rat mesothelial cells co-express vimentin and the simple epithelial cytokeratins. While cytokeratins predominate in situ, under most culture conditions vimentin is the major intermediate filament protein of the cells. This loss of cytokeratin production upon culture can be partly prevented by growing mesothelial cells on a basement membrane matrix. However, the basement membrane-promoted persistence of cytokeratin synthesis is not accompanied by expression of cytokeratin G (no. 19), the major acidic cytokeratin of mesothelium in vivo. While cells grown on plastic establish a prominent juxtanuclear assemblage of tonofilaments, those cultured on basement membrane exhibit cytokeratin filaments which are distributed throughout the cytoplasm and attach to neighboring cells at the plasma membrane. This latter pattern resembles that seen in the intact mesothelium. Intermediate filaments are markers of cellular differentiation, but their roles are obscure. The response of cultured mesothelial cells to different growth substrata supports the hypothesis that intermediate filament synthesis is influenced by cellular contact with the extracellular matrix.
大鼠间皮细胞共表达波形蛋白和简单上皮细胞角蛋白。虽然细胞角蛋白在原位占主导，但在大多数培养条件下，波形蛋白是细胞的主要中间丝蛋白。通过在基底膜基质上培养间皮细胞，可部分防止培养时细胞角蛋白产生的丢失。然而，基底膜促进的细胞角蛋白合成持续性并不伴随着细胞角蛋白G（第19号）的表达，细胞角蛋白G是体内间皮的主要酸性细胞角蛋白。虽然在塑料上生长的细胞形成了明显的核周张力丝聚集，但在基底膜上培养的细胞表现出细胞角蛋白丝，其分布在整个细胞质中并在质膜处与相邻细胞相连。后一种模式类似于在完整间皮中看到的模式。中间丝是细胞分化的标志物，但其作用尚不清楚。培养的间皮细胞对不同生长基质的反应支持了中间丝合成受细胞与细胞外基质接触影响的假说。
