Allegrucci C, Young L E
School of Human Development, University of Nottingham, Queens Medical Centre, Nottingham, UK.
Hum Reprod Update. 2007 Mar-Apr;13(2):103-20. doi: 10.1093/humupd/dml041. Epub 2006 Aug 26.
The promise of human embryonic stem cell (hESC) lines for treating injuries and degenerative diseases, for understanding early human development, for disease modelling and for drug discovery, has brought much excitement to scientific communities as well as to the public. Although all of the lines derived worldwide share the expression of characteristic pluripotency markers, many differences are emerging between lines that may be more associated with the wide range of culture conditions in current use than the inherent genetic variation of the embryos from which embryonic stem cells were derived. Thus, the validity of many comparisons between lines published thus far is difficult to interpret. This article reviews the evidence for differences between lines, focusing on studies of pluripotency marker molecules, transcriptional profiling, genetic stability and epigenetic stability, for which there is most evidence. Recognition and assessment of environmentally induced differences will be important to facilitate the development of culture systems that maximize stability in culture and provide lines with maximal potential for safety and success in the range of possible applications.
人类胚胎干细胞（hESC）系在治疗损伤和退行性疾病、理解人类早期发育、疾病建模以及药物发现等方面的前景，给科学界和公众都带来了极大的兴奋。尽管全球所有衍生的细胞系都表达特征性多能性标志物，但不同细胞系之间正出现许多差异，这些差异可能更多地与当前广泛使用的培养条件有关，而非与胚胎干细胞所源自胚胎的固有遗传变异有关。因此，迄今为止发表的许多细胞系间比较的有效性难以解释。本文回顾了细胞系间存在差异的证据，重点关注多能性标志物分子、转录谱分析、遗传稳定性和表观遗传稳定性的研究，这些方面有最多的证据。识别和评估环境诱导的差异对于促进培养系统的发展很重要，该培养系统能在培养中最大限度地提高稳定性，并为细胞系在一系列可能应用中提供最大的安全和成功潜力。
