Tindle R W, Smith J A, Geysen H M, Selvey L A, Frazer I H
Department of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
J Gen Virol. 1990 Jun;71 ( Pt 6):1347-54. doi: 10.1099/0022-1317-71-6-1347.
Human papillomavirus (HPV) type 16 is implicated in the aetiology of anogenital dysplasia which may progress to malignancy. HPV-16 DNA is actively transcribed in cervical carcinomas, the most abundant transcripts being from the E6 and E7 early open reading frames. The E7 protein has been shown to have transforming activity in vitro. In this report we define four immunodominant B epitopes within the protein corresponding to the E7 gene, using a panel of murine monoclonal antibodies. Three epitopes are linear and lie within the N-terminal region of the molecule, and are unique to the HPV-16 E7 protein. One epitope is non-linear and presumed to be conformational. At least three of the four epitopes of the E7 protein are detectable by immunoprecipitation from an HPV-16-infected cervical carcinoma cell line. The demonstrated immunogenicity of the E7 protein allows us to deduce that this molecule may be a potential candidate for incorporation in a vaccine against cervical cancer.
16型人乳头瘤病毒（HPV）与肛门生殖器发育异常的病因有关，这种发育异常可能会发展为恶性肿瘤。HPV - 16 DNA在宫颈癌中活跃转录，最丰富的转录本来自E6和E7早期开放阅读框。E7蛋白已被证明在体外具有转化活性。在本报告中，我们使用一组鼠单克隆抗体，确定了该蛋白中与E7基因相对应的四个免疫显性B表位。三个表位是线性的，位于分子的N端区域，是HPV - 16 E7蛋白所特有的。一个表位是非线性的，推测为构象性表位。通过对HPV - 16感染的宫颈癌细胞系进行免疫沉淀，可以检测到E7蛋白的四个表位中至少三个。E7蛋白所显示的免疫原性使我们推断，该分子可能是宫颈癌疫苗的潜在候选成分。
