Kyndt T, Droogenbroeck B Van, Haegeman A, Roldán-Ruiz I, Gheysen G
Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Genome. 2006 Jul;49(7):786-98. doi: 10.1139/g06-035.
To generate inexpensive and efficient DNA markers for addressing a number of population genetics problems and identification of wild hybrids in Vasconcellea, we have evaluated the use of simple sequence repeat (SSR) primers previously developed for other species. A set of 103 Vasconcellea accessions and some individuals of the related genera Carica and Jacaratia were analyzed with 10 primer pairs directing amplification of chloroplast microsatellites in Nicotiana tabacum and 9 nuclear SSR primer pairs recently identified in Vasconcellea x heilbornii. Heterologous amplification of chloroplast SSRs was successful for 8 of the 10 loci, of which 6 showed polymorphism. Seven of the 9 nuclear SSR primer pairs were useful in Vasconcellea and often also in Jacaratia and Carica, all revealing polymorphism. Exclusive haplotypes for each described taxon were identified based on chloroplast microsatellite data. Clustering based on separate nuclear and chloroplast data resulted in a clear grouping per taxon, but only low resolution was obtained above species level. The codominancy of nuclear SSRs and the general high polymorphism rate of SSR markers will make them more useful in future population genetics studies and diversity assessment in conservation programs.
为了开发廉价且高效的DNA标记，以解决一些群体遗传学问题并鉴定番木瓜属中的野生杂种，我们评估了先前为其他物种开发的简单序列重复（SSR）引物的使用情况。使用10对指导烟草叶绿体微卫星扩增的引物以及最近在番木瓜属×海尔博尼番木瓜中鉴定出的9对核SSR引物，对103份番木瓜属种质以及相关属番荔枝属和加拉提亚属的一些个体进行了分析。叶绿体SSRs的异源扩增在10个位点中的8个位点上成功，其中6个显示出多态性。9对核SSR引物中的7对在番木瓜属中有用，在加拉提亚属和番荔枝属中通常也有用，均显示出多态性。根据叶绿体微卫星数据确定了每个所述分类群的独特单倍型。基于单独的核数据和叶绿体数据进行聚类，每个分类群都有明显的分组，但在物种水平以上仅获得了低分辨率。核SSRs的共显性和SSR标记普遍较高的多态性率将使它们在未来的群体遗传学研究和保护计划中的多样性评估中更有用。
