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[在营养培养基上对大肠杆菌毒力的测定]

[The determination of Escherichia virulence on nutrient media].

作者信息

Grigor'eva L V, Malakhova L A, Kolesnikov V G

出版信息

Mikrobiol Zh (1978). 1990 Jan-Feb;52(1):65-9.

PMID:1693750
Abstract

The virulence of 250 pathogenic Escherichia strains secreted from humans and from various environmental objects has been studied on nutrient media with stains, ram erythrocytes and in hemagglutination reactions. The highest percentage of strain virulence is revealed when using crystal violet and in the blood agar (44.0 and 27.2%, respectively), evidently, due to nonspecific reactions. The medium with congo red showed a positive result in 20.3 + 3.1%, and the d-mannose-resistant hemagglutination reaction--in 16.5 + 2.3% cases. In practice it is recommended to use several methods which enable estimating correctly the risk to infect humans and to conduct timely preventive measures.

摘要

对从人类和各种环境物体中分离出的250株致病性大肠杆菌菌株,在含有染色剂、公羊红细胞的营养培养基上以及在血凝反应中进行了毒力研究。使用结晶紫和血琼脂时,菌株毒力的百分比最高(分别为44.0%和27.2%),显然这是由于非特异性反应。刚果红培养基显示阳性结果的比例为20.3%±3.1%,而d - 甘露糖抗性血凝反应显示阳性结果的比例为16.5%±2.3%。在实际操作中,建议使用多种方法,以便正确评估感染人类的风险并及时采取预防措施。

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