血小板膜糖蛋白Ib：在完整分子及其蛋白水解片段中检测到的基因多态性。

Platelet membrane glycoprotein Ib: genetic polymorphism detected in the intact molecule and in proteolytic fragments.

作者信息

Meyer M, Schellenberg I

机构信息

Department of Medical Genetics, Medical Academy of Erfurt, G.D.R.

出版信息

Thromb Res. 1990 May 1;58(3):233-42. doi: 10.1016/0049-3848(90)90093-r.

DOI:10.1016/0049-3848(90)90093-r

PMID:1693792

Abstract

Genetic polymorphism of platelet membrane glycoprotein (GP) Ib was studied in 128 healthy German blood donors using immunoblotting with monospecific anti-glycocalicin antibodies. Only three GP Ib forms (B,C and D) were detected. In a smaller number of Vietnamese individuals analysed for comparison all four polymorphic GP Ib forms were found. The frequency distribution of GP Ib phenotypes in the German population was similar to that reported for U.S. Americans of Caucasian origin. GP Ib polymorphism was also observed in purified glycocalicin samples of single donors. Using trypsin-cleaved glycocalicin the polymorphic region could be located in the C-terminal carbohydrate-rich glycocalicin fragment (macroglycopeptide).

摘要

利用单特异性抗糖萼蛋白抗体进行免疫印迹法，对128名健康德国献血者的血小板膜糖蛋白（GP）Ib的基因多态性进行了研究。仅检测到三种GP Ib形式（B、C和D）。在为作比较而分析的少量越南个体中，发现了所有四种多态性GP Ib形式。德国人群中GP Ib表型的频率分布与报道的白种裔美国人群相似。在单个供体的纯化糖萼蛋白样本中也观察到了GP Ib多态性。利用胰蛋白酶切割的糖萼蛋白，多态性区域可定位于富含碳水化合物的C端糖萼蛋白片段（大糖肽）。