免疫调节寡核苷酸在预防卵清蛋白诱导的咽鼓管功能障碍中的作用。
The role of immunomodulatory oligonucleotides in prevention of OVA-induced Eustachian tube dysfunction.
作者信息
Ebert Charles S, Rose Austin S, Patel Mihir R, Hardy Stuart M, Kandimalla Ekambar R, Agrawal Sudhir, Prazma Jiri, Pillsbury Harold C
机构信息
Department of Otolaryngology-Head and Neck Surgery, CB# 7070, G0412 Neurosciences Hospital, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7070, United States.
出版信息
Int J Pediatr Otorhinolaryngol. 2006 Dec;70(12):2019-26. doi: 10.1016/j.ijporl.2006.07.016. Epub 2006 Aug 30.
OBJECTIVE
To evaluate the potential role of immunomodulatory oligonucleotides (IMO) in the prevention of OVA-induced Eustachian tube dysfunction (ETD) in a rat model.
METHODS
Brown-Norway rats were sensitized to ovalbumin (OVA) and randomized to receive pre-treatment with IMO or phosphate buffered saline (PBS). After systemic sensitization, subjects received a transtympanic OVA challenge followed by evaluation of the Eustachian tube's dynamic function.
RESULTS
Pre-treatment of OVA sensitized animals with IMO normalized passive opening and closing Eustachian tube pressures, improved active clearance of negative pressure in the middle ear, and resulted in reduced mean mucociliary transit times compared to untreated OVA-sensitized animals (P<0.001).
CONCLUSION
These data demonstrate that pre-treatment with IMO prevent OVA-induced ETD in the rat. IMO treatment in the future may offer considerable promise in the management of OME in children.
目的
在大鼠模型中评估免疫调节寡核苷酸（IMO）在预防卵清蛋白（OVA）诱导的咽鼓管功能障碍（ETD）中的潜在作用。
方法
将棕色挪威大鼠对卵清蛋白（OVA）进行致敏，并随机分为接受IMO预处理或磷酸盐缓冲盐水（PBS）预处理的组。全身致敏后，对受试者进行经鼓膜OVA激发，随后评估咽鼓管的动态功能。
结果
与未治疗的OVA致敏动物相比，用IMO对OVA致敏动物进行预处理可使咽鼓管被动开闭压力正常化，改善中耳负压的主动清除，并导致平均黏液纤毛转运时间缩短（P<0.001）。
结论
这些数据表明，IMO预处理可预防大鼠中OVA诱导的ETD。未来IMO治疗可能在儿童中耳炎的管理中具有很大的前景。
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