[广东省女性肿瘤坏死因子-β基因多态性与子宫内膜异位症的相关性]

[Association of tumor necrosis factors-beta gene polymorphism with endometriosis in women in Guangdong Province].

作者信息

Luo Min, He Yuan-li, Zhang Hong-bin, Shen Dong-xiang, Zong Li-li, Guan Ting

机构信息

Department of Obstetrics and Gynecology, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2006 Aug;26(8):1163-5.

PMID:16939911

Abstract

OBJECTIVE

To study the polymorphism of +252 site in intron 1 of tumor necrosis factor (TNF)-beta gene in relation to genetic susceptibility of endometriosis.

METHODS

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to detect the polymorphism of +252 site in intron 1 of TNF-beta gene in 82 Chinese Han patients with endometriosis in Guangdong Province and 80 Han patients without endometriosis (control group), and the relation between TNF gene polymorphism and the risk of endometriosis was analyzed.

RESULTS

The +252 site of TNF-beta allele and genotype distribution showed significant difference between endometriosis and control groups (Chi2=6.562, P<0.05; chi2=6.562, P<0.05), and relative risk of endometriosis in relation to allele A was increased by 1.793 fold. The risk of endometriosis was 3.33-fold higher in women of AA genotype than those of GG genotype (Chi2=6.562, P<0.05).

CONCLUSIONS

Allele A in TNF-beta gene +252 site can significantly increase the relative risk of endometriosis in women in Guangdong, among which TNF-beta AA genotype might be one of the genetic susceptible factors for endometriosis.

摘要

目的

研究肿瘤坏死因子（TNF）-β基因第1内含子+252位点多态性与子宫内膜异位症遗传易感性的关系。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测82例广东省汉族子宫内膜异位症患者及80例无子宫内膜异位症的汉族患者（对照组）TNF-β基因第1内含子+252位点的多态性，并分析TNF基因多态性与子宫内膜异位症发病风险的关系。

结果

TNF-β等位基因及基因型在子宫内膜异位症组与对照组中的分布差异有统计学意义（χ²=6.562，P<0.05；χ²=6.562，P<0.05），等位基因A导致子宫内膜异位症的相对风险增加1.793倍。AA基因型女性患子宫内膜异位症的风险是GG基因型女性的3.33倍（χ²=6.562，P<0.05）。