Hasegawa Akiko, Kanazawa Nozomi, Sawai Hideaki, Komori Shinji, Koyama Koji
Laboratory of Developmental Biology and Reproduction, Hyogo College of Medicine, Institute for Advanced Medical Sciences, Nishinomiya, Japan.
Reproduction. 2006 Sep;132(3):455-64. doi: 10.1530/rep.1.01016.
The zona pellucida, an extracellular matrix surrounding mammalian oocytes, is composed of three or four glycoproteins. It is well known that the zona pellucida plays several critical roles during fertilization, but there is little knowledge about its formation. The purpose of this study is to examine whether a pig zona pellucida glycoprotein 2 (pZP2) would assemble with mouse zona pellucida. A transgene construct was prepared by placing a minigene encoding pZP2 downstream from the promoter of mouse ZP2. The result showed that the transgenic protein was synthesized in growing oocytes but not incorporated into the zona pellucida. Furthermore, the pZP2 transgene did not rescue the phenotype in ZP2-knockout zona-deficient mice. These results indicate that pZP2 does not participate in mouse zona pellucida formation and the zona pellucida is constituted from its component proteins in a molecular species-specific manner between mice and pigs.
透明带是围绕哺乳动物卵母细胞的一种细胞外基质，由三种或四种糖蛋白组成。众所周知，透明带在受精过程中发挥着几个关键作用，但关于其形成的了解却很少。本研究的目的是检测猪透明带糖蛋白2（pZP2）是否会与小鼠透明带组装。通过将编码pZP2的小基因置于小鼠ZP2启动子的下游来制备转基因构建体。结果显示，转基因蛋白在生长中的卵母细胞中合成，但未整合到透明带中。此外，pZP2转基因未能挽救ZP2基因敲除的无透明带小鼠的表型。这些结果表明，pZP2不参与小鼠透明带的形成，并且透明带是以小鼠和猪之间分子物种特异性的方式由其组成蛋白构成的。
