Ottavio L, Chang C D, Rizzo M G, Petralia S, Travali S, Baserga R
Department of Pathology, Temple University Medical School, Philadelphia, PA 19140.
Biochem Biophys Res Commun. 1990 Jun 15;169(2):509-16. doi: 10.1016/0006-291x(90)90360-y.
mRNA levels for the Proliferating Cell Nuclear Antigen (PCNA) gene are growth regulated. PCNA promoters of different lengths were used to drive a linked reporter, the cDNA for human thymidine kinase (TK). After transfection into TK ts13 cells, stable cell lines were obtained. Regardless of promoter length, in all cell lines the levels of TK mRNA were roughly similar in serum-deprived and serum stimulated cells, confirming, by an independent method, that the growth regulation of PCNA mRNA levels doe not depend on the 5' flanking sequence of the PCNA gene.
增殖细胞核抗原（PCNA）基因的mRNA水平受生长调节。使用不同长度的PCNA启动子驱动一个连接的报告基因，即人胸苷激酶（TK）的cDNA。转染到TK ts13细胞后，获得了稳定的细胞系。无论启动子长度如何，在所有细胞系中，血清剥夺和血清刺激的细胞中TK mRNA水平大致相似，通过一种独立的方法证实，PCNA mRNA水平的生长调节不依赖于PCNA基因的5'侧翼序列。
