Borca M V, Moore D M, Srikumaran S, Morgan D O
Department of Veterinary Science, University of Nebraska, Lincoln.
Viral Immunol. 1990 Summer;3(2):147-60. doi: 10.1089/vim.1990.3.147.
Foot-and-mouth disease virus (FMDV) capsid contains 60 copies each of four structural proteins, virus proteins 1-4. Virus protein 1 (VP1) plays an important immunogenic role, being the only VP that is immunogenic as an isolated protein. Even peptides representing a partial amino acid (AA) sequence of VP1 can induce protective immunity in experimental hosts. A 32 AA residue, in a tandem repeat configuration (32dimer), of sero/subtype A-12 Lp ab VP1 (AA 132-168) was highly immunogenic for its homologous subtype and partially protective for FMDV serotype A strain A24. This cross-reactivity was further demonstrable in the ELISA and mouse protection tests. Three different antibody populations were isolated by affinity chromatography (AFC) from the serum of a steer immunized with the 32dimer. Each population seems to recognize a different epitope on the 32dimer peptide since each fraction was defined as unique by its reactivity with different subtypes of FMDV virus in RIA, ELISA, neutralization and competition assays. Considering the neutralizing activity of each of the antibody populations the pattern of neutralization of the 32dimer elicited antiserum can be described. Two of the three epitopes were mapped by competition assays using synthetic peptides.
口蹄疫病毒（FMDV）衣壳包含四种结构蛋白（病毒蛋白1-4）各60个拷贝。病毒蛋白1（VP1）发挥着重要的免疫原性作用，是唯一一种作为分离蛋白具有免疫原性的病毒蛋白。即便只是代表VP1部分氨基酸（AA）序列的肽段，也能在实验宿主中诱导保护性免疫。A-12 Lp ab VP1（第132-168位氨基酸）的一个呈串联重复结构（32二聚体）的32个氨基酸残基对其同源亚型具有高度免疫原性，对A24型口蹄疫病毒血清型毒株具有部分保护作用。这种交叉反应性在ELISA和小鼠保护试验中得到了进一步证实。通过亲和色谱法（AFC）从用32二聚体免疫的公牛血清中分离出三种不同的抗体群体。每个群体似乎识别32二聚体肽段上不同的表位，因为在放射免疫分析（RIA）、ELISA、中和及竞争试验中，每个组分与不同亚型的口蹄疫病毒的反应性都被定义为独特的。考虑到每个抗体群体的中和活性，可以描述32二聚体诱导的抗血清的中和模式。通过使用合成肽的竞争试验对三个表位中的两个进行了定位。
