Hou K C, Zaniewski R
CUNO, Inc., Life Sciences Division, Meriden, Connecticut 06450.
Biotechnol Appl Biochem. 1990 Jun;12(3):315-24.
The effects of anion-exchange polymeric matrices on endotoxin removal from albumin and gamma-globulin solutions are evaluated. The positively charged cellulose acrylic media carrying DEAE or QAE functional groups remove significant amounts of endotoxin from tap water, but are less effective in protein solutions. With properly controlled pH levels and salt concentrations, the endotoxin level in a protein solution can be reduced; however, low endotoxin concentrations, less than 100 pg/ml, are more difficult to remove. The endotoxin removal capacity depends on the number of functional groups existing in the matrix, expressed as the number of milliequivalents (meq), and on the pH operable range, which is directly related to the pK alpha value of the matrix. The effects of pH and salt on endotoxin removal from albumin and gamma-globulin solutions by an anion-exchange polymeric matrix were evaluated statically in test tubes. In addition, a dynamic flow was performed under statically defined conditions on a 250-ml DEAE cartridge for the removal of endotoxin from albumin at a flow rate of 40 ml/min. A greater than 75% reduction in the endotoxin can be achieved, with protein loss occurring only in the early stage of removal. Such processes are useful for the reduction of endotoxin from biological solutions produced by natural sources or recombinant DNA technology.
评估了阴离子交换聚合物基质对从白蛋白和γ-球蛋白溶液中去除内毒素的效果。带有DEAE或QAE官能团的带正电荷的纤维素丙烯酸介质能从自来水中去除大量内毒素，但在蛋白质溶液中的效果较差。在适当控制的pH值和盐浓度下，蛋白质溶液中的内毒素水平可以降低；然而，低于100 pg/ml的低内毒素浓度更难去除。内毒素去除能力取决于基质中存在的官能团数量（以毫当量数表示）以及与基质的pKα值直接相关的可操作pH范围。通过在试管中静态评估pH值和盐对阴离子交换聚合物基质从白蛋白和γ-球蛋白溶液中去除内毒素的影响。此外，在静态定义的条件下，以40 ml/min的流速在250 ml DEAE柱上进行动态流动，以从白蛋白中去除内毒素。内毒素可降低75%以上，且蛋白质损失仅发生在去除的早期阶段。此类方法对于降低天然来源或重组DNA技术生产的生物溶液中的内毒素很有用。
