Takata T, Nikai H, Miyauchi M, Ito H, Kobayashi J, Ijuhin N
Department of Oral Pathology, Hiroshima University School of Dentistry, Japan.
J Periodontal Res. 1990 May;25(3):152-5. doi: 10.1111/j.1600-0765.1990.tb01037.x.
Oral gingival epithelium (OGE), oral sulcular epithelium (OSE) and junctional epithelium (JE) were examined histochemically by using different lectins as markers for epithelial differentiation. The staining pattern of gingival epithelia was compared with that of the buccal and palatal epithelia. Binding of WGA had a uniform distribution in all the epithelia examined. A positive reaction was found in all the basal and spinous layers, but not in the cornified layer of the epithelia. BPA binding was seen in the lower spinous layer of OGE, OSE, buccal and palatal epithelia, and in most of the JE. The basal layer and the cells directly attached to the tooth surface at the apical part of JE were nonreactive with BPA. GS-I reacted with the basal and suprabasal layers of each epithelium and with the cells attached to the tooth at the apical part of JE. UEA-I reacted with the upper spinous layer of OGE, OSE and epithelia of hard palate, but not with any of the cells of the JE. Our results agree with previous data suggesting that OGE and OSE exhibit squamous differentiation similar to that of the masticatory epithelium of hard palate. Furthermore, our results suggested that the JE cells undergo differentiation equivalent to that of the suprabasal and lower spinous cells of OGE. The cells along the tooth surface at the apical part of JE, however, form a distinct population of cells with basal nature.
采用不同凝集素作为上皮分化标志物，对口腔牙龈上皮（OGE）、口腔龈沟上皮（OSE）和结合上皮（JE）进行了组织化学检查。将牙龈上皮的染色模式与颊上皮和腭上皮的染色模式进行了比较。WGA在所有检查的上皮中结合分布均匀。在所有上皮的基底层和棘层均发现阳性反应，但在角化层未发现。在OGE、OSE、颊上皮和腭上皮的棘层下层以及大部分JE中可见BPA结合。JE顶端的基底层和直接附着于牙面的细胞对BPA无反应。GS-I与各上皮的基底层和基底层上层以及JE顶端附着于牙的细胞发生反应。UEA-I与OGE、OSE和硬腭上皮的棘层上层发生反应，但不与JE的任何细胞发生反应。我们的结果与先前的数据一致，表明OGE和OSE表现出与硬腭咀嚼上皮相似的鳞状分化。此外，我们的结果表明，JE细胞经历的分化与OGE的基底层上层和棘层下层细胞相当。然而，JE顶端沿牙面的细胞形成了一个具有基底性质的独特细胞群。
