Picanço-Diniz D L, Valença M M, Franci C R, Antunes-Rodrigues J
Department of Physiology, School of Medicine, University of São Paulo, Ribeirão Preto, Brazil.
Neuroendocrinology. 1990 Jun;51(6):675-82. doi: 10.1159/000125409.
The present studies were designed to evaluate the role of substance P (SP) in the control of the release of luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin (PRL). SP was microinjected into the medial preoptic area (MPOA) of conscious, freely moving intact or orchidectomized (ORCX; 21 days post-ORCX) adult male rats. Microinjection of SP into the MPOA induced a significant decrease in plasma LH and FSH concentrations, effects which were accompanied by an elevation in plasma PRL concentration. To examine the participation of endogenously secreted SP in the activity of the MPOA neurons controlling release of these cited pituitary hormones, another study was performed in which either a potent and specific antagonist to SP (D-Pro2, D-Trip7,9-SP; SP-ANT) or an antibody against SP (SP-AB), was injected into the MPOA. SP-ANT and SP-AB both elevated plasma LH, FSH and decreased plasma PRL concentration. These data suggest that endogenous SP within the MPOA exerts an important inhibitory tonus over LH and FSH release and an excitatory tonus over PRL release. In conclusion, SP seems to participate as a neurotransmitter or neuromodulater in the control of LH, FSH and PRL secretion, at least in part, by acting at the level of MPOA, a region in which the neuronal cell bodies that produce LH-releasing hormone and the associated gonadotropin-releasing hormone-associated peptide are located.
本研究旨在评估P物质（SP）在控制促黄体生成素（LH）、促卵泡激素（FSH）和催乳素（PRL）释放中的作用。将SP微量注射到清醒、自由活动的成年完整雄性大鼠或去势（ORCX；ORCX后21天）雄性大鼠的内侧视前区（MPOA）。向MPOA微量注射SP可导致血浆LH和FSH浓度显著降低，同时血浆PRL浓度升高。为了研究内源性分泌的SP在控制上述垂体激素释放的MPOA神经元活动中的参与情况，进行了另一项研究，将一种强效且特异性的SP拮抗剂（D-脯氨酸2，D-色氨酸7,9-SP；SP-ANT）或抗SP抗体（SP-AB）注射到MPOA中。SP-ANT和SP-AB均使血浆LH、FSH升高，并使血浆PRL浓度降低。这些数据表明，MPOA内的内源性SP对LH和FSH释放具有重要的抑制性紧张作用，对PRL释放具有兴奋性紧张作用。总之，SP似乎至少部分地通过作用于MPOA水平，作为神经递质或神经调节剂参与LH、FSH和PRL分泌的控制，MPOA是产生促黄体激素释放激素和相关促性腺激素释放激素相关肽的神经元细胞体所在的区域。
