Schipper H M, Scarborough D E, Lechan R M, Reichlin S
Department of Neurology, McGill University, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis--Jewish General Hospital, Montreal, Que., Canada.
Brain Res Dev Brain Res. 1990 Jun 1;54(1):71-9. doi: 10.1016/0165-3806(90)90066-8.
Gomori-positive astrocytes have been identified in the periventricular brain in situ and in diencephalic explants on the basis of their endogenous peroxidase activity, affinity for chrom alum hematoxylin, and orange-red autofluorescence. To facilitate analyses of their functional properties, we sought to identify these cells in dissociated fetal rat brain cultures. Astrocytes containing cytoplasmic inclusions with the above tinctorial and fluorescent properties represented less than 1% of cultured astrocytes at day 10 in vitro (DIV). There was a marked increase in the fraction of Gomori-positive astrocytes and their granule content between 10 and 46 DIV. As in situ, the peroxidase activity appeared to be non-enzyme-mediated insofar as it catalyzed diaminobenzidine oxidation over a wide range of pH (3-11) and could not be inhibited by tissue preheating or the catalase inhibitor, aminotriazole. Metalloporphyrins probably mediate both the pseudoperoxidase activity and autofluorescence in these cells. Cysteamine and cystamine, but not ethanolamine or L-cysteine, induced a massive accumulation of Gomori-positive astrocytes when administered from DIV 6-18. Alterations of the redox microenvironment or induction of porphyrin/heme biosynthetic enzymes may be the mechanisms responsible for this cyst(e)amine effect. Dissociated rat brain culture enriched for Gomori astroglia should provide ample opportunity to investigate the functional properties of these cells.
基于内源性过氧化物酶活性、对铬明矾苏木精的亲和力以及橙红色自发荧光，已在脑室周围脑原位和间脑外植体中鉴定出戈莫里阳性星形胶质细胞。为便于分析其功能特性，我们试图在解离的胎鼠脑培养物中鉴定这些细胞。在体外培养第10天（DIV）时，含有具有上述染色和荧光特性的细胞质内含物的星形胶质细胞占培养星形胶质细胞的比例不到1%。在10至46 DIV之间，戈莫里阳性星形胶质细胞及其颗粒含量的比例显著增加。与原位情况一样，过氧化物酶活性似乎不是由酶介导的，因为它在很宽的pH范围（3 - 11）内催化二氨基联苯胺氧化，并且不能被组织预热或过氧化氢酶抑制剂氨基三唑抑制。金属卟啉可能介导这些细胞中的假过氧化物酶活性和自发荧光。从DIV 6 - 18给药时，半胱胺和胱胺而非乙醇胺或L - 半胱氨酸可诱导戈莫里阳性星形胶质细胞大量积累。氧化还原微环境的改变或卟啉/血红素生物合成酶的诱导可能是这种半胱（胺）效应的机制。富含戈莫里星形胶质细胞的解离大鼠脑培养物应提供充足机会来研究这些细胞的功能特性。
