Kuchler K, Richter K, Trnovsky J, Egger R, Kreil G
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
J Biol Chem. 1990 Jul 15;265(20):11731-3.
From the skin of Xenopus laevis, the cloned cDNAs of two precursors of thyrotropin-releasing hormone have been isolated and sequenced. These encode almost identical precursor polypeptides each containing seven copies of the end product flanked by typical prohormones processing signals. Northern blot analysis has corroborated the existence of two thyrotropin-releasing hormone precursor mRNAs of similar size as the cloned cDNA and demonstrated the existence of a third smaller species as well. Two or more mRNAs for this precursor are also present in the brain and eyes of X. laevis adults and tadpoles.
从非洲爪蟾的皮肤中，已分离并测序了促甲状腺激素释放激素两种前体的克隆cDNA。这些cDNA编码几乎相同的前体多肽，每种多肽都含有七个终产物拷贝，两侧是典型的激素原加工信号。Northern印迹分析证实存在两种与克隆cDNA大小相似的促甲状腺激素释放激素前体mRNA，同时也证明了还存在第三种较小的mRNA。这种前体的两种或更多种mRNA也存在于非洲爪蟾成体和蝌蚪的大脑和眼睛中。
