Bailey Trina R, Paulsen Daniel B, Sehgal Inder, Hosgood Giselle
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Am J Vet Res. 2006 Sep;67(9):1628-34. doi: 10.2460/ajvr.67.9.1628.
To determine distribution of urokinase plasminogen activator-like protein and urokinase plasminogen activator receptor-like protein in urinary tract tissues of healthy dogs.
11 healthy dogs.
Necropsy specimens from kidney, ureter, bladder, urethra, prostate, and testis were obtained from 4 sexually intact female dogs, 5 sexually intact males, and 2 castrated males; dogs ranged in age from juvenile to adult. Urokinase plasminogen activator-like protein and urokinase plasminogen activator receptor-like protein in tissue lysates from kidney, prostate, and testis were identified by use of SDS-PAGE, western blot analysis, and immunoprecipitation. Urokinase plasminogen activator-like protein and urokinase plasminogen activator receptor-like protein in kidney, ureter, urinary bladder, urethra, prostate, and testis were identified by use of immunohistochemical staining of tissue sections.
Urokinase plasminogen activator-like protein and urokinase plasminogen activator receptor-like protein in the molecular-weight range published for urokinase and urokinase receptor (53 and 33 kd for urokinase and 60 to 65 kd for urokinase receptor) were identified. Distribution of the proteins identified by use of immunohistochemical staining was comparable with published information for humans and mice for the urinary tract. Staining of these proteins was detected in more tissue types than reported in healthy humans.
Urokinase plasminogen activator-like protein and urokinase plasminogen activator receptor-like protein were detected in the urinary tract of healthy dogs. This information is important for further evaluation of the functions of urokinase and urokinase receptor in the canine urinary tract and the pathophysiologic features of urinary tract disease.
确定健康犬泌尿道组织中尿激酶型纤溶酶原激活物样蛋白和尿激酶型纤溶酶原激活物受体样蛋白的分布情况。
11只健康犬。
从4只性成熟雌性犬、5只性成熟雄性犬和2只去势雄性犬获取肾脏、输尿管、膀胱、尿道、前列腺和睾丸的尸检标本；犬的年龄范围从幼年到成年。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、蛋白质免疫印迹分析和免疫沉淀法鉴定肾脏、前列腺和睾丸组织裂解物中的尿激酶型纤溶酶原激活物样蛋白和尿激酶型纤溶酶原激活物受体样蛋白。通过组织切片的免疫组织化学染色鉴定肾脏、输尿管、膀胱、尿道、前列腺和睾丸中的尿激酶型纤溶酶原激活物样蛋白和尿激酶型纤溶酶原激活物受体样蛋白。
鉴定出分子量在已公布的尿激酶和尿激酶受体范围内的尿激酶型纤溶酶原激活物样蛋白和尿激酶型纤溶酶原激活物受体样蛋白（尿激酶为53和33kd，尿激酶受体为60至65kd）。通过免疫组织化学染色鉴定的这些蛋白质的分布与已公布的人类和小鼠泌尿道信息相当。在比健康人类报道的更多组织类型中检测到了这些蛋白质的染色。
在健康犬的泌尿道中检测到尿激酶型纤溶酶原激活物样蛋白和尿激酶型纤溶酶原激活物受体样蛋白。该信息对于进一步评估尿激酶和尿激酶受体在犬泌尿道中的功能以及泌尿道疾病的病理生理特征具有重要意义。
