通过EGTA诱导的钙耗竭抑制盘基网柄菌中的聚集。

Inhibition of aggregation in Dictyostelium by EGTA-induced depletion of calcium.

作者信息

Europe-Finner G N, McClue S J, Newell P C

机构信息

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.

出版信息

FEMS Microbiol Lett. 1984 Jan;21(1):21-5. doi: 10.1111/j.1574-6968.1984.tb00179.x.

DOI:10.1111/j.1574-6968.1984.tb00179.x

PMID:16948639

Abstract

During aggregation of Dictyostelium discoideum, amoebae move towards collecting centres emitting cyclic AMP. Previous work has indicated that Ca2+ can affect the operation of various parts of the chemotactic mechanism but there are contradictory reports about the role or requirement for Ca2+ during aggregation of intact amoebae. In this study we show that there is a requirement for Ca2+ during this aggregation process. Addition of EGTA (7 mM) and in some cases extensive washing with EGTA is needed to see inhibition of aggregation. Considerable stores of Ca2+ present within cells may explain the failure of some previous attempts to see a Ca2+ requirement during aggregation.

摘要

在盘基网柄菌聚集过程中，变形虫会朝着释放环磷酸腺苷的收集中心移动。先前的研究表明，钙离子可以影响趋化机制各个部分的运作，但关于完整变形虫聚集过程中钙离子的作用或需求，存在相互矛盾的报道。在本研究中，我们表明在这个聚集过程中需要钙离子。添加乙二醇双四乙酸（7 mM），在某些情况下用乙二醇双四乙酸进行大量洗涤，才能观察到聚集受到抑制。细胞内存在的大量钙离子储备可能解释了先前一些观察聚集过程中钙离子需求的尝试为何失败。