He Ying-li, Zhao Ying-ren, Zhang Shu-lin, Yang Yuan, Liu Min, Jin Yan, Liu Jin-feng
Infectious Department, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Sep;22(5):620-2.
To investigate peripheral blood mononuclear cells (PBMCs) immune-related gene expression profile in patients with chronic virus hepatitis B(CHB) by oligonucleotide gene array technique.
PBMCs were collected from the members of a family of clustering hepatitis B virus (HBV) infection including 5 CHB patients and 4 healthy spouses and RNA prepared from PBMCs was hybridized to high-density oligonucleotide arrays(HG-U133A 2.0 Human Gene Chips, Affymetrix), covering the expression of 22 000 human ESTs. Primary scanned image was analyzed with DNT software package.
Out of the 22 000 ESTs, 24 different immune-related genes were identified. Among the 24 genes, 7 genes showed increased expression and 17 genes showed decreased expression in CHB compared with those in healthy spouses. The up-regulated genes were mainly associated with adaptive immunity, while the down-regulated genes were associated with innate immunity.
Our findings suggest that HBV infection alters a broad range of immunity genes expression and innate immunity-associated genes are important in the defense against HBV chronic infection.
采用寡核苷酸基因芯片技术研究慢性乙型病毒性肝炎（CHB）患者外周血单个核细胞（PBMCs）免疫相关基因表达谱。
从一个聚集性乙型肝炎病毒（HBV）感染家族成员中采集PBMCs，该家族包括5例CHB患者和4名健康配偶，从PBMCs中提取的RNA与覆盖22000个人类ESTs表达的高密度寡核苷酸芯片（HG-U133A 2.0人类基因芯片，Affymetrix公司）进行杂交。用DNT软件包分析初次扫描图像。
在22000个ESTs中，鉴定出24个不同的免疫相关基因。与健康配偶相比，CHB患者中这24个基因里有7个基因表达增加，17个基因表达下降。上调基因主要与适应性免疫相关，而下调基因与固有免疫相关。
我们的研究结果提示，HBV感染改变了广泛的免疫基因表达，且固有免疫相关基因在抵御HBV慢性感染中很重要。
