Liem R S, van Willigen J D, Copray J C, Ter Horst G J
Department of Neurobiology and Oral Physiology, Medical and Dental School, University of Groningen, The Netherlands.
Acta Anat (Basel). 1990;138(1):65-74. doi: 10.1159/000146921.
The source of innervation of the corpuscular bodies in the palate and the central projections of the afferent fibres of the entire palate was studied in rats by transganglionic transport of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP) and with substance P (SP) immunohistochemistry. WGA-HRP injected into the incisal papilla was taken up by the nerve fibres that terminated in the corpuscles. Retrogradely labelled neurons were observed in the trigeminal ganglion as well as anterogradely labelled terminals in the dorsolateral part of the spinal trigeminal nucleus and in the lateral part of the nucleus of the solitary tract. No labelling could be found in the geniculate ganglion, the facial nerve and the hypoglossal nucleus. Following WGA-HRP injection in the intermolar area and in the soft palate, labelling was only restricted to the trigeminal ganglion. The lamina propria of the entire palate and the corpuscle-enriched area of the incisal papilla and the soft palate were richly innervated by SP-containing fibres. Numerous SP-containing fibres were also observed in the nerve plexus at the base of the corpuscle. In addition, SP-positive neurons were identified in the trigeminal ganglion and SP-labelled terminals in the sensory trigeminal nuclear complex and in the solitary tract nucleus. On the basis of our morphological observations we conclude that the palatal corpuscular bodies are involved in taste perception which is of trigeminal origin.
采用结合了小麦胚凝集素的辣根过氧化物酶（WGA-HRP）跨神经节运输法以及P物质（SP）免疫组织化学方法，在大鼠中研究了腭部小体的神经支配来源以及整个腭部传入纤维的中枢投射。将WGA-HRP注入切牙乳头，终末于小体的神经纤维摄取该物质。在三叉神经节中观察到逆行标记的神经元，同时在三叉神经脊束核背外侧部和孤束核外侧部观察到顺行标记的终末。在膝状神经节、面神经和舌下神经核中未发现标记。在磨牙间区域和软腭注射WGA-HRP后，标记仅局限于三叉神经节。整个腭部的固有层以及切牙乳头和软腭富含小体的区域有丰富的含SP纤维支配。在小体基部的神经丛中也观察到许多含SP纤维。此外，在三叉神经节中鉴定出SP阳性神经元，在感觉三叉神经核复合体和孤束核中观察到SP标记的终末。基于我们的形态学观察，我们得出结论，腭部小体参与三叉神经起源的味觉感知。
