来自大肠杆菌孔蛋白OmpF的合成肽段构成白细胞激活剂。
Synthetic peptide segments from the Escherichia coli porin OmpF constitute leukocyte activators.
作者信息
Vordermeier H M, Hoffmann P, Gombert F O, Jung G, Bessler W G
机构信息
Institut für Immunobiologie der Universität, Freiburg, Federal Republic of Germany.
出版信息
Infect Immun. 1990 Aug;58(8):2719-24. doi: 10.1128/iai.58.8.2719-2724.1990.
Abstract
After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-285) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differentiation into antibody-producing plasma cells and tumor cytotoxicity of macrophages against the fibroblast cell line L929. We thus demonstrated that defined peptide segments are responsible for the leukocyte-activating properties of a major bacterial surface protein.
摘要
在对孔蛋白OmpF进行表征并通过计算机辅助表位分析选择负责白细胞激活的分子结构后，合成并测试了类似物OmpF（153 - 174）（包含氨基酸153至174）、OmpF（157 - 174）和OmpF（275 - 285）。与天然蛋白质一样，这些片段对BALB/c脾细胞具有促有丝分裂作用，并诱导B淋巴细胞分化为产生抗体的浆细胞，以及巨噬细胞对成纤维细胞系L929的肿瘤细胞毒性。因此，我们证明了特定的肽段负责一种主要细菌表面蛋白的白细胞激活特性。
相似文献
1
Synthetic peptide segments from the Escherichia coli porin OmpF constitute leukocyte activators.来自大肠杆菌孔蛋白OmpF的合成肽段构成白细胞激活剂。
Infect Immun. 1990 Aug;58(8):2719-24. doi: 10.1128/iai.58.8.2719-2724.1990.
2
Identification of murine B-cell and T-cell epitopes of Escherichia coli outer membrane protein F with synthetic polypeptides.用合成多肽鉴定大肠杆菌外膜蛋白F的小鼠B细胞和T细胞表位
Infect Immun. 2000 May;68(5):2535-45. doi: 10.1128/IAI.68.5.2535-2545.2000.
3
Immunological evidence for differences in the exposed regions of OmpF porins from Escherichia coli B and K-12.来自大肠杆菌B和K-12的OmpF孔蛋白暴露区域差异的免疫学证据。
Mol Immunol. 1988 Jun;25(6):555-63. doi: 10.1016/0161-5890(88)90077-6.
4
Determinants of OmpF porin antigenicity and structure.外膜孔蛋白F（OmpF）抗原性和结构的决定因素。
J Biol Chem. 1990 Apr 25;265(12):6800-10.
5
Immunological analysis of porin polymorphism in Escherichia coli B and K-12.大肠杆菌B和K-12中孔蛋白多态性的免疫学分析。
Mol Immunol. 1989 Nov;26(11):1027-36. doi: 10.1016/0161-5890(89)90067-9.
6
The orientation of porin OmpF in the outer membrane of Escherichia coli.孔蛋白OmpF在大肠杆菌外膜中的取向。
J Mol Biol. 1993 Oct 5;233(3):400-13. doi: 10.1006/jmbi.1993.1520.
7
Structural relatedness of enteric bacterial porins assessed with monoclonal antibodies to Salmonella typhimurium OmpD and OmpC.用抗鼠伤寒沙门氏菌OmpD和OmpC单克隆抗体评估肠道细菌孔蛋白的结构相关性。
J Bacteriol. 1992 Mar;174(6):1965-73. doi: 10.1128/jb.174.6.1965-1973.1992.
8
Immunological comparison of major outer membrane proteins from different strains of Escherichia coli.不同大肠杆菌菌株主要外膜蛋白的免疫学比较。
Ann Inst Pasteur Microbiol. 1987 Jul-Aug;138(4):393-406. doi: 10.1016/0769-2609(87)90057-3.
9
Identification and characterization of T helper cell epitopes of the major outer membrane protein of Chlamydia trachomatis.沙眼衣原体主要外膜蛋白T辅助细胞表位的鉴定与特性分析
J Exp Med. 1990 Jul 1;172(1):203-12. doi: 10.1084/jem.172.1.203.
10
Effect of lipopolysaccharide structure on reactivity of antiporin monoclonal antibodies with the bacterial cell surface.脂多糖结构对抗孔蛋白单克隆抗体与细菌细胞表面反应性的影响。
J Bacteriol. 1988 Mar;170(3):1063-8. doi: 10.1128/jb.170.3.1063-1068.1988.
引用本文的文献
1
Major outer membrane protein Omp25 of Brucella suis is involved in inhibition of tumor necrosis factor alpha production during infection of human macrophages.猪布鲁氏菌的主要外膜蛋白Omp25在感染人类巨噬细胞期间参与抑制肿瘤坏死因子α的产生。
Infect Immun. 2001 Aug;69(8):4823-30. doi: 10.1128/IAI.69.8.4823-4830.2001.
2
Identification of murine B-cell and T-cell epitopes of Escherichia coli outer membrane protein F with synthetic polypeptides.用合成多肽鉴定大肠杆菌外膜蛋白F的小鼠B细胞和T细胞表位
Infect Immun. 2000 May;68(5):2535-45. doi: 10.1128/IAI.68.5.2535-2545.2000.
3
Electrophoretic analysis of the major outer membrane protein of Chlamydia psittaci reveals multimers which are recognized by protective monoclonal antibodies.鹦鹉热衣原体主要外膜蛋白的电泳分析显示出多聚体，这些多聚体可被保护性单克隆抗体识别。
Infect Immun. 1995 Jun;63(6):2387-9. doi: 10.1128/iai.63.6.2387-2389.1995.
4
Synthetic peptides analogous to the fimbrillin sequence inhibit adherence of Porphyromonas gingivalis.与菌毛蛋白序列类似的合成肽可抑制牙龈卟啉单胞菌的黏附。
Infect Immun. 1992 Apr;60(4):1662-70. doi: 10.1128/iai.60.4.1662-1670.1992.
本文引用的文献
1
Endotoxic properties of chemically synthesized lipid A part structures. Comparison of synthetic lipid A precursor and synthetic analogues with biosynthetic lipid A precursor and free lipid A.
Eur J Biochem. 1984 Apr 16;140(2):221-7. doi: 10.1111/j.1432-1033.1984.tb08090.x.
2
Protein antigen conformation: folding patterns and predictive algorithms; selection of antigenic and immunogenic peptides.蛋白质抗原构象：折叠模式与预测算法；抗原性和免疫原性肽段的选择。
Ann Sclavo Collana Monogr. 1984;1(2):47-60.
3
Three-dimensional structure of membrane and surface proteins.膜蛋白和表面蛋白的三维结构。
Annu Rev Biochem. 1984;53:595-623. doi: 10.1146/annurev.bi.53.070184.003115.
4
Synthesis of the mitogenic S-[2,3-bis(palmitoyloxy)propyl]-N-palmitoylpentapeptide from Escherichia coli lipoprotein.源自大肠杆菌脂蛋白的促有丝分裂S-[2,3-双（棕榈酰氧基）丙基]-N-棕榈酰五肽的合成。
Hoppe Seylers Z Physiol Chem. 1983 May;364(5):593-606. doi: 10.1515/bchm2.1983.364.1.593.
5
6
Prediction of protein antigenic determinants from amino acid sequences.从氨基酸序列预测蛋白质抗原决定簇。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8. doi: 10.1073/pnas.78.6.3824.
7
Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides.脂多糖在体外诱导免疫球蛋白和抗体合成
Eur J Immunol. 1972 Aug;2(4):349-53. doi: 10.1002/eji.1830020410.
8
Specific antibodies elicited by antigen covalently linked to a synthetic adjuvant.由与合成佐剂共价连接的抗原引发的特异性抗体。
Immunobiology. 1985 Sep;170(3):239-44. doi: 10.1016/S0171-2985(85)80095-4.
9
10
A synthetic analogue of Escherichia coli lipoprotein, tripalmitoyl pentapeptide, constitutes a potent immune adjuvant.大肠杆菌脂蛋白的合成类似物，三棕榈酰五肽，是一种有效的免疫佐剂。
J Immunol. 1986 Oct 15;137(8):2676-81.
Zotero 插件