Filbin M T, Tennekoon G I
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
J Neurochem. 1990 Aug;55(2):500-5. doi: 10.1111/j.1471-4159.1990.tb04163.x.
The major PNS myelin protein, P0, has been expressed in Chinese hamster ovary cells by transfection with a plasmid containing the P0-cDNA. The expression of P0 at both the RNA and the protein level was greatly increased, without detriment to the cell, by the dihydrofolate reductase-methotrexate strategy of gene amplification. The P0 expressed by these cells was glycosylated (containing approximately equal amounts of the complex and high-mannose type glycoproteins) and reached the plasma membrane. This system is suitable not only for addressing the function of P0 directly, but it also applicable to any protein of which an abundance is needed.
主要的外周神经系统髓鞘蛋白P0，已通过用含有P0 - cDNA的质粒转染中国仓鼠卵巢细胞而得以表达。通过二氢叶酸还原酶 - 甲氨蝶呤基因扩增策略，P0在RNA和蛋白质水平的表达均大幅增加，且对细胞无害。这些细胞表达的P0发生了糖基化（含有大致等量的复合型和高甘露糖型糖蛋白）并到达了质膜。该系统不仅适用于直接研究P0的功能，也适用于任何需要大量表达的蛋白质。
