Mossberg K, Ericsson M
Royal Institute of Technology, Stockholm, Sweden.
J Microsc. 1990 May;158(Pt 2):215-24. doi: 10.1111/j.1365-2818.1990.tb02995.x.
Studies of doubly stained specimens were performed with a confocal scanning microscope. The instrument used provides the possibility of making separate detections of the fluorescent dyes. The optimal choice of excitation wavelengths and optical filters are discussed. The fluorphores that were used are Lucifer Yellow, Texas Red, fluorescein isothiocyanate and tetramethylrhodamine isothiocyanate.
对双重染色的标本进行了共聚焦扫描显微镜研究。所使用的仪器提供了分别检测荧光染料的可能性。讨论了激发波长和光学滤光片的最佳选择。所使用的荧光团有荧光素黄、德克萨斯红、异硫氰酸荧光素和异硫氰酸四甲基罗丹明。
