Dietzschold B, Gore M, Marchadier D, Niu H S, Bunschoten H M, Otvos L, Wunner W H, Ertl H C, Osterhaus A D, Koprowski H
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104-4268.
J Virol. 1990 Aug;64(8):3804-9. doi: 10.1128/JVI.64.8.3804-3809.1990.
We have mapped a linear epitope recognized by the virus-neutralizing monoclonal antibody 6-15C4 within the primary sequence of the G protein from the Evelyn-Rokitnicki-Abelseth strain of rabies virus. This was accomplished by using fragments of the rabies virus G protein and deduced amino acid sequences of neutralization-resistant variant rabies viruses. The monoclonal antibody 6-15C4 specifically recognized a synthetic peptide (peptide G5-24) which resembles the 6-15C4 epitope in structure. In addition, a tandem peptide constructed from the G5-24 peptide and a dominant TH cell epitope of the rabies virus N protein induced protective immunity against lethal rabies virus challenge infection in mice.
我们已经在狂犬病病毒伊夫林-罗基特尼基-阿贝尔塞斯毒株G蛋白的一级序列中定位了病毒中和单克隆抗体6-15C4识别的线性表位。这是通过使用狂犬病病毒G蛋白片段和抗中和变异狂犬病病毒的推导氨基酸序列来完成的。单克隆抗体6-15C4特异性识别一种合成肽（肽G5-24），其结构类似于6-15C4表位。此外，由G5-24肽和狂犬病病毒N蛋白的显性TH细胞表位构建的串联肽在小鼠中诱导了针对致死性狂犬病病毒攻击感染的保护性免疫。
