Macfarlan R I, Dietzschold B, Wiktor T J, Kiel M, Houghten R, Lerner R A, Sutcliffe J G, Koprowski H
J Immunol. 1984 Nov;133(5):2748-52.
The antigenic structure of the rabies virus glycoprotein has been studied. A limited number of fragments were obtained by cyanogen bromide (CNBr) cleavage of viral glycoprotein, and eight large peptides were isolated by using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. These were tested for their capacity to stimulate the proliferation of nylon wool-purified T cells obtained from spleens of rabies-immune A/J mice. Three peptides (Cr1, Cr2 plus Cr2A, and Cr3) stimulated antigen-specific proliferation, indicating that at least three T cell determinants of the native molecule are sequential or continuous in nature. Stimulation was also obtained with 27-residue and 13-residue synthetic peptides (designated R21 and R20, respectively) that included sequences towards the carboxy terminal end of Cr1, but not with synthetic peptides that included sequences of Cr2 and Cr3 (which are both glycosylated in virus-derived material). The intact viral glycoprotein and synthetic peptide R21 stimulated T lymphocytes with surface characteristics of helper cells, and induced the production of interleukin 2 by these lymphocytes. Synthetic peptides R20 and R21 also stimulated a minor population of Lyt-2-positive cells, which were not yet identified as either suppressor or cytotoxic T lymphocytes.
已对狂犬病病毒糖蛋白的抗原结构进行了研究。通过用溴化氰（CNBr）裂解病毒糖蛋白获得了数量有限的片段，并使用十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳分离出8个大肽段。检测了它们刺激从狂犬病免疫的A/J小鼠脾脏中获得的经尼龙毛纯化的T细胞增殖的能力。三种肽段（Cr1、Cr2加Cr2A和Cr3）刺激了抗原特异性增殖，表明天然分子的至少三个T细胞决定簇在本质上是连续的。含Cr1羧基末端序列的27个残基和13个残基的合成肽（分别命名为R21和R20）也能刺激增殖，但含Cr2和Cr3序列（在病毒衍生材料中均被糖基化）的合成肽则不能。完整的病毒糖蛋白和合成肽R21刺激具有辅助细胞表面特征的T淋巴细胞，并诱导这些淋巴细胞产生白细胞介素2。合成肽R20和R21还刺激了一小部分Lyt-2阳性细胞，这些细胞尚未被鉴定为抑制性或细胞毒性T淋巴细胞。
