Grebenshchikova O G, Rashkovetskiĭ L G, Prozorovskiĭ V N
Lab Delo. 1990(4):59-61.
The authors compare the methods for immobilization of porcine lactate dehydrogenase-5 (LDH5) on various carriers to be used for the preparation of an effective immunosorbent for the isolation of monospecific antibodies. Covalent binding of the enzyme with the carrier via a spacer grouping with the use of glutaraldehyde is advisable for LDH M4-isoform that is characterized by a quaternary structure.
作者比较了将猪乳酸脱氢酶-5（LDH5）固定在各种载体上的方法，这些方法用于制备一种有效的免疫吸附剂以分离单特异性抗体。对于具有四级结构特征的LDH M4同工型，通过使用戊二醛经间隔基团使酶与载体共价结合是可取的。
